AggiiUiudfion Ted in Abortion. 373 



ff/ji/iosus. Smears from plat'ontal membi-am.' showed streptobacilli 

 and strept(K(tcri. as above. Neither specimen showed bacilli which 

 tduhl be detiiutely recognised as tlie l)acilli of contagious abortion. 

 Cii/fiirex of the 7i. ahortns were not obtained, the tubes being over- 

 grown by the other organisms present. 



III. Cow, " Baptist."— Aborted on 22nd June, 1914. Exudate, 

 secured from the vagina two days later, gave agglutination with 

 0.0025 CO. Microscopical ejamination of c.rudate showed numerous 

 organisms morphologically like B. abort ax. 



Inoculation of Aniniah. — Guinea-pig \'t^^ was inoculated sub- 

 cutaneously with exudate, and its serum, tested nine days later, 

 gave no agglutination ; 10 days after irjoculation a positive agglu- 

 tination was obtained with 0.15 c.c. of serum; 76 days after inocu- 

 lation 0.0025 c.c. of serum gave a positive agglutination; 95 days 

 after inocidation the animal was killed. 



I'ost-morteni E.raniinafion. — Animal in good condition and all 

 organs apparently normal, except the spleen, which showed a nodu- 

 lar appearance externally and on section numerous very small 

 greyish foci, which looked rather more like enlarged and prominent 

 malpighian bodies than foci of necrotic material. On smears, no 

 organisms could be detected. 



Guinea-pig 489, inoculated intraperitoneally with exudate, was 

 tested 40 days after inoculation, when the serum gave a positive 

 agglutination reaction. 



IV. Cow. "Garfish." — Calved 5th July, 1914, not being due 

 till the I7tli of the same month, but the owner remarks: "Calf 

 full size, but dead." Exudate w'as secured two days after parturi- 

 tion, and gave no agglutination when tested, using 0.05 c.c. 



Microscopical era mi not ion of exudate failed to demonstrate the 

 presence of B. abortus, and ciiltures gave negative results. 



Animals Inoculated. — Guinea-pig 491, inoculated intraperitone- 

 ally with exudate, was tested three times (the last test ll.'i days 

 after inoculation), the serum giving no agglutination. Guinea-pig 

 492, inoculated subcutaneously with exudate, tested one month 

 after inoculation, gave a similar negative residt. 



Whey from milk collected eight days after calving, gave a nega- 

 tive result with 0.05 c.c. 



2. Technique Employed. 



In the following pages details of the technique employed in the 

 use of all the materials examined are given with a view to a 



