228 JIHEI KONISHI AND YNGVE ZOTTERMAN 



The infrared analyses of both the above mentioned ether soluble and 

 acetone insoluble fractions of pupa demonstrated absorption bands which 

 are characteristic for glycerophospholipid. The spectrum possesses strong 

 bands of ester C = stretching, P=-0 linkage and other bands which may 



Fig. U. Showing the selective adaptation. I- A, response to human saliva ; 

 I — B, response to the application of the saliva following previous adaptation 

 to milk ; II — A, response to silkworm pupa fluid, II — B, response to the 

 application of the pupa fluid following previous adaptation to human saliva. 



Time in 0.5 sec. 



Fig. 12. Electrical response from the carp's palatine nerve to the application 



of lecithine to the palatal organ. A, pupa fluid ; B, 0.5 per cent lecithine from 



yolk ; C, 0.5 per cent lecithine from soya bean. Time in 0.5 sec. 



serve as reference peaks for the identification of glycerophospholipids 

 (Freeman et al., 1953 ; Marinetti and Stotz, 1954), but lacks the absorption 

 bands which are assigned to monosubstituted amide groups. On the other 

 hand, the spectrum from the ether insoluble fraction showed strong absorp- 

 tion bands of the monosubstituted amide group and very strong OH and 



