I2 4 



RESEARCHES ON FUNGI 



remained for hours, and sometimes for days, between 0° and 3° C. 

 By opening the outer door for a few minutes the air of the room 

 could easily be reduced to 0° C, and this temperature was often 

 maintained for several hours. 



So far experiments have been limited to species which grow 

 upon wood and have proved capable of withstanding uninjured 

 the prolonged and severe frost of the Manitoban winter. Dried 

 fruit-bodies of Lenzites betulina, Poly st ictus versicolor, P. hirsutus, 

 Dmdalea unicolor, and Schizophyllum commune were placed in 

 a damp-chamber with wet cotton-wool upon the upper surfaces 



of their pilei. 

 They soon re- 

 vived, and at the 

 end of six hours, 

 upon being ex- 

 amined with a 

 beam of light, 

 they were found 

 to be vigorously 

 shedding spores. 

 A fruit-body, to 



Fig. 46. — Apparatus for demonstrating the fall of spores from ^q tested was 

 fruit-bodies at 0° C. A glass dish u is placed on a wooden 



shelf w in the cold-room. An inverted glass dish i is packed taken to the 



round with snow s so as to leave the space within it unfilled. . , , 



To the cork c is attached the fruit-body/, below which is Cold-room and 



placed a glass slide g for the purpose of catching the falling I) : nne( j to „ cor k 



spores. About \ actual size. " ' 



attached by 

 means of sealing-wax to the bottom of a small crystallising dish. This 

 was then inverted and packed round with melting snow contained 

 in another and much larger crystallising dish, as shown in Fig. 46. 

 After two hours, when the fruit- body had become cooled to freezing 

 point, the cold-room air was reduced to 0° C. by opening the outer 

 door for a few minutes. The inverted crystallising dish, to which 

 the fruit-body was attached, was then lifted out of the snow, so 

 that fresh spore-free air at 0° C. entered it. A glass slide (Fig. 46,^) 

 was then placed so that when the crystallising dish was replaced 

 in position, the fruit-body had its natural orientation, its under, 

 spore-producing surface looking directly down on the glass slide. 



