Proclaetlon of Antlsero, 53 



Uhlenhuth (15, XI. 1900) was able to obtain antiscnnn in a rabbit 

 Jed on ogg-white, the solution being introduced daily by means of a 

 sound, and the precipitin appearing after the feeding had been con- 

 tnuied 24 days. Sunilarly Michaelis and Oppenheimcr (1902, p. 855) 

 led rabbits with ox serum in large quantities, and obtained antiserum 

 for ox blood. They attribute the formation of antiserum, through 

 excessive feeding with heterologous albumen, to the escape of some of it 

 from the action of the peptic digestion, for precipitins for milk etc are 

 not found m the serum of normally fed man. 



In the following table I have summarized the methods of treatment 

 of rabbits adopted by different workers, the data in some eases beiiu^ 

 rather incomplete. 



Antiserum 

 for blood 

 or milk of 



Man 



Mode of i 4"iO""'' in- i Total 

 injection J^cted at a ; amount in- 

 tnne in c.c. i jected in c.c. 



Ox 



Cow-milk 

 etc. 



Human al- 

 buminous 

 urine J 



subcut. 

 perit. 



venous 

 perit. 



10 



5—10 

 10 



1 

 10 



subcut. 10—50 



perit. 

 venous 



10 

 7 

 20 



50—60 



80—200 



6 

 50—60 



30 



56 



150—200 



Duration of Day wlien 

 treatment I bled after 

 in days i last injection ! 



Autlior and Reference 

 with date 



14 



14—21 

 16—40 



8 



30—40 



21 



7 



28 

 90 



6 ? Wassermann and 

 Schiitze, 18. ii. 01 



Stern, 28. ii. 01 



Kister and Wolff, 

 : 18. xi. 02 



7 Strube, 12. vi. 02 

 Uhlenliutb, 7. i. 01 



6 Wassermann and 



;' Schiitze, 1900-19U1 



7 I Moro, 31. X. 01 



I Uhlenhuth, 6. xii. 02 

 15 I Leclainche and 

 i Vallee, 25. i. 01 



* Gave reaction with 1 : 1000 blood dilutions. 



t Milk sterilized 90 minutes at 65^ C, injected every 4 days. 



J Contained 1—2 g. o| albumen per litre. 



Uhlenhuth made injecticms every 5-6 days. Stern every two days 

 Wassermann and Schutze every 3-4 days, others at intervals which 

 are generally not stated. In Bordet's experiments (ill. 1899 p 240 

 not cited m the table), the milk was partially sterilised at 65^' C prior 

 to injection intraperitoneally. Wassermann and Schiitze sterilized it 

 by means of chloroform. 



Without making an attempt at completeness, the above table very 

 clearly shows a great divergence in the mode of treatment, more especially 

 with regard to the duration of treatment and the amounts of substance 



