Effects on different Albumins 107 



Hamburger (cited by Aschoff, 1902, p. 193) obtained precipitins for 

 casein and albumin of cows' milk, stating that he was able to differentiate 

 the one from the other, although both antisera precipitated ox serum, 

 whereas ox haematoserum did not att'ect milk. 



Michaelis and Oppenheimer (1902, p. 343), working with anti-ox 

 serum, euglobulin and pseudoglobulin, found these antisera to act upon 

 globulins but not upon albumin. Both acted more upon pseudoglobulin 

 than upon euglobulin, the action of anti-euglobulin being markedly stronger 

 with euglobulin than that of anti-pseudoglobulin. Anti-ox haematoserum 

 acted on ox serum-globulin, pseudoglobulin, somewhat less on euglobulin, 

 slightly or not at all on serum-albumin. They consider (p. 345) these 

 antisera not " specific " for each albumin of the same animal, although 

 they certainly react with some more than with others. They conclude 

 therefore that the specific " combining groups " are not possessed by 

 one form of albuminous molecule, but that they are common to 

 chemically related albumins. The precipitin reactions are therefore 

 of no use for the qualitative chemical separation of the different 

 albumins of the same animal. 



Rostoski (1902, a.) treated rabbits with horse serum, as also with 

 horse serum-globulin, euglobulin, pseudoglobulin and serum-albumin, 

 but found that all the antisera reacted with the different serum 

 constituents above named. As I have stated elsewhere, this might 

 be ascribed to his using milky antisera (see p. 73) which unfortunately 

 robs his results of their value. A rabbit treated with Bence-Jones 

 albumins gave an antiserum which acted upon these, but also upon 

 human serum, serum-albumin and globulin, but not with the serum or 

 serum derivatives of other animals. Like the preceding authors he 

 concludes that precipitins afford no aid in distinguishing the albumins 

 of the same animal. In a subsequent paper Rostoski (1902, h., p. 21) 

 sums up the results of Leblanc and of Hamburger and points out their 

 obvious contradictions. These are evident from what I have stated 

 above. 



Umber (14, vii. 1902) on the whole confirms the results of 

 Obermayer and Pick, Michaelis, and Rostoski. He treated rabbits 

 with egg-albumin and globulin solutions and tested egg-white and 

 the homologous substances with the antisera produced. Separating 

 fibrinogen, globulin and albumin from the antisera, he dissolved them 

 in saline and tested them upon the egg-white constituents named. 

 Anti-globulin and anti-albumin serum precipitated dilutions of egg- 

 white, of globulins, but not of crystallized egg-albumin. The separated 



