180 Tests ivith Precipitins 



I found it to produce marked clouding in a number of mammalian 

 bloods: that of man, several species of monkey, bear, dog, opossum, 

 raccoon, cat, coati, genet, stoat, rat, mouse. It did not however produce 

 a full reaction with any blood except that of the pig, no other suilline 

 bloods being tested. Only once did the antiserum produce a slight 

 clouding in an avian blood, this being attributed to experimental error, 

 the sample in question having probably been in contact with some 

 mammalian blood, through carelessness in collection. Still later, 

 (5, IV. 1902), I observed faint cloudings to occur upon the addition of 

 this antiserum to the bloods of the antelope and deer, and after 80 to 

 120 minutes in the bloods of bats (6 species), 3 species of Edentates, and 

 8 species of Australian Marsupials. I also drew attention to the 

 interesting fact that the blood of the porpoise immediately gave a slight 

 clouding with this antiserum, a well-marked though slight deposit being 

 formed after 24 hours. I Avrote at the time that " The more general 

 action of this particular anti-pig serum on other mammalian bloods may 

 at first appear to contradict what has hitherto been claimed with regard 

 to the relatively specific character of these antisera; but this is 

 actually not the case, I have already noted elsewhere the occasional 

 occurrence of clouding in non-homologous bloods upon the addition of an 

 antiserum." I have attributed these results to the great power of my 

 antisera, giving what I have described elsewhere as a " mammalian 

 reaction." 



Besides Uhlenhuth and myself, only Kister and Wolff (18, XI. 1902, 

 p. 422) are known to me as having experimented with anti-pig serum. 

 They state that their antiserum did not act on human blood in any 

 concentration, and this I attribute to their antiserum not being as 

 powerful as mine. Under the same conditions the antiserum gave a 

 reaction in 5 minutes with pig blood (followed by a large deposit in 

 1 — 2 hours), a cloud in the sera of ox and sheep after 20 minutes, and 

 in the horse after 60 minutes. 



Finally, Schlitze (6, xi. 1902, p. 805) found pig haematoserum to 

 precipitate solutions of pig spermatozoa, but not those of man. 



818 Tests with Anti-Pig Serum. 



Three different antisera were used in the following tests, the three 

 having been obtained by injecting as many rabbits with the serum of 

 the domestic pig. All three antisera were exceedingly powerful, two of 



