188 



Tests with Precipitins 



Zoological Gardens, London. The antiserum was very powerful, giving 

 a preci})itum of "055 when standardized. 



We see from the above table that the large reactions are limited to 

 the Bovidae, the second-class reactions to Bovidae (21 7o) ^^^ Ccrvidae 

 (81 7o of their totals). Two negative results amongst the Pecora are 

 referred to in footnotes. Other Artiodactyla than the Pecora gave 71 7o 

 total reactions. Hyrax gave a faint clouding, the Equidae negative 

 results. The Cetacea also gave negative residts, although it is stated in 

 the tables that a trace of deposit was noted in the tubes after 24 hours. 

 Of the non-ungulate bloods those of the Primates and Carnivora give 

 most reaction. 



(7) Tests with Anti-Ox Serum. 



The first to produce anti-ox serum was the late Dr Walter Myers 

 (14, VII. 1900) who treated rabbits with ox serum-globulin, obtaining 

 an antiserum which precipitated solutions of ox globulin, besides having 

 a slight action on a similar solution from sheep. Uhlenhuth (1, li. 1901) 

 produced it next by subjecting rabbits to intraperitoneal injections of 

 defibrinated ox blood, doses of about 10 c.c. being administered every 

 6 — 8 days until 5 injections had been made. He tested 19 different 

 bloods therewith in 1 : 200 dilutions. The bloods tested were the 

 following, which I have grouped in order: 



Presumably his anti-ox serum was weak, for he states that he 

 obtained a reaction only with ox blood. In my preliminary note of 

 11, V. 1901, in which tests on 24 bloods were reported, I noted that 

 anti-ox serum also produced a weak reaction with the blood of the sheep. 

 As the number of bloods tested increased, I found that those of the 

 gazelle and axis-deer gave distinct reactions (1, vii. 1901), as did also 

 those of the goat, roebuck, and Burrhel sheep (14, ix. 1901), and in 



