Auti-Sheei) Serum 191 



reactions than did the blood of the sheep. Uhlenhuth (25, vii. 1901) 

 tested 24 bloods (sec list p. 172) with this antiserum, finding that it 

 gave almost as much reaction with the blood of tho goat as with that of 

 the sheep, less with that of the ox. He observed no reaction in the 

 blood of a deer, nor in any of the other bloods tested. With an in- 

 creasing number of bloods tested, I noted more and more the tendency 

 of this antiserum to include other Bovidae in its positive reactions, and 

 not infrequently Cervidae. In several short papers these findings were 

 briefly dwelt upon as in the preceding tests with anti-ox serum. 



Recently Schlitze (6, xi. 1902, p. 805) has found anti-sheep serum 

 to act less upon ox spermatozoa solutions than upon corresponding ones 

 of the sheep. The results of Kister and Wolff (18, xi. 1902, p. 421) 

 with this antiserum are in accord with mine, and of themselves indicate 

 that an experimental error lay at the bottom of those they obtained 

 with anti-ox serum referred to above. Anti-sheep serum clouded both 

 sheep and ox serum dilutions in 5 minutes, leading to a large deposit 

 in the former after 2 hours. After 20 to 30 minutes slight clouds 

 appeared in pig and horse serum. No effect was exerted on human 

 blood after 2 hours. As the proportion of antiserum added to the 

 dilution decreased, the blood of the pig and horse ceased to react, 

 reactions still being obtained with the bloods of sheep and ox. 



701 Tests with Anti-Sheep Serum. 



Six different antisera, obtained from as many rabbits, were used for 

 these tests. The rabbits received injections of the serum of the domesti- 

 cated sheep. One of the antisera had moderate power, whereas the 

 others were powerful. One of the latter gave a precipitum of '02 when 

 standardized. 



Here again the large reactions are limited to the bloods of Bovidae 

 (23 7o)> the second-class reactions occur amongst Bovidae and Cervidae, 

 forming a higher percentage of the total reactions in the latter group. 

 Of the bloods of other Ungulata, those of Suina and Equidae gave faint 

 and medium cloudings. One of two cetacean bloods tested gave a 

 medium clouding. Outside the Ungulata, those of Primates and 

 Carnivora gave most of the "mammalian reactions" recorded, viz. 

 5 and 10 7o respectively. Two of the four bloods of Pecora giving 

 negative results were from India. 



