NuttalVs Method 315 



1. Antisera for Primates : Man (2 series) 



Ourang 



2. ,, Inscctivora : Hedgehog 



3. „ Carnivora : Cat 



Dog 



4. ,, Ungidata : Sheep (3 series) 



Ox 



Antelope (Cobus unctuosus) 



Cariacus mexicanus 



Cervus porcinus 



Eangifer tarandus 



Pig (3 series) 



Horse -, 



Donkey I several series 



Zebra (E. Grevyi)) 



5. ,, Marsupialia : Ooychogale unguifera 



6. „ Aves : Struthio molybdophanes 



Gallus domesticus 



Before proceeding to describe the different series of tests it appears 

 desirable to give briefly the method of testing quantitatively, quoting 

 Nuttall's description which appeared in the British Medical Journal for 

 April 5th, 1902, since his original method has not been modified. 



Method for the Measurement of the Degree of Reaction. 



" Dilutions of the serums to be tested quantitatively are made with 

 0"6 per cent, normal salt solution to the degree desired — namely, 1 : 100, 

 1 : 200, etc. The solutions must be perfectly clear and free from 

 suspended matter. Accurately-measured quantities — usually 0*5 cm. 

 of the dilution — are transferred into a series of clean, dry test-tubes, 

 having a capacity of about 1 cm and a lumen of about 5 mm. The 

 dilution is allowed to run into the tubes, along a fine, freshly-drawn, 

 sealed capillary tube in order to avoid the formation of bubbles or of 

 overflow in filling the tubes. Each tube is supplied with a sealed 

 capillary, which is left in place after the introduction of the dilution; 

 subsequently serving a similar purpose when adding the antiserum. 

 An accurately-measured quantity of antiserum is now added to the 

 dilution, in the proportion of 20 : 1 to 200 : 1 or more of the blood in 

 dilution. After the antiserum has been added to the series of tubes 

 the capillaries are removed and the mixture of dilution and antiserum 

 is effected. This can be done very satisfactorily by applying the tip of 

 the clean finger to the mouth of the tube and shaking it up and down. 



