344 Anflsera for Birds Efjcfs 



Seven tests were carried out with dilutions uf Reptile egg'-aibuinin.s 

 of which 28 ^o were positive. 



No positive reactions were obtained with 10, and 25 tests, respect- 

 ively with Amphibian, and Fish egg-albumins. 



The 215 tests with Re})tilian sera yielded noteworthy results. All 

 the antisera tested reacted to some extent with Chelonian liloods, but 

 only anti-fowl's egg serum (I) gave any positive results with the other 

 Reptilia. 



Out oi' 64 experiments with Amphibian sera only (jue showed any 

 trace of reaction, namely, a very feeble clouding when anti-fowl's-egg 

 serum was added to the diluted blood of the Toad. 



180 tests with Fish, and 46 with Crustacean sera gave no indications 

 of positive reactions. 



These experiments, therefore, indicate a relationship between the 

 Aves and Chelonia, and a less marked one between the Aves and 

 Crocodilia, As indicated by this test the relationship between the 

 Aves and Lacertilia and Ophidia is very remote (see pp. 200 — 207, 

 21(3, 300—305). 



C. Quantitative Measm'ements with Antisera to 

 Birds Erjg-alhundn. 



These experiments were conducted by the method which has already 

 been described (p. 315), and the results of the measurements of precipita 

 in each case are stated in c.c. in the second coKunn. The i»ercentages 

 given in the last cohunn are calculated on the basis of the amount 

 of precii)itum obtained, the amount given by an antiserum with its 

 homologous blood being taken as 100*^/^. In most cases this figure 

 has not been exceeded when the antiserum acted on a non-homologous 

 blood. 



