(Mrahain-Smlth ;U7 



and Sihcr Pheasant cgg-alhuniins, marked ivactinns with (Jrecntincli, 

 Moorhen, and Hedge-sparrow, and small with Knni. 



The quantitative nietliod fails to bring out the delicate reactions 

 which can be detei-mined by the qualitative method, but in the case of 

 the major reactions ditit'ei-enees can bc^ recognised which are not apparent 

 by the latter method. By its means closely allied sera and egg-albumins 

 can be differentiated, whicli cannot be done with certainty by the quali- 

 tative method unless low dilutions arc' made use of. The results of the 

 few quantitative experiments which have been matie are exactly in 

 accord with those obtained by the qualitative method. 



II. Antisera for Reptilia. 



Qualitative Tests with Anti-Reptilian Sera. 



A. Anti-Chelonian Sera. 



(1) Tests 'With Anti-Tortoise Seraiu. 



This antiserum was pre})ared by intraperitoneal injections of the 

 serum of the Tortoise {Test ado ihera) into a Rabbit. The serum used 

 was obtained from healthy tortoises. 



Material tested • 

 26 F.gg-AIbionins 



((/) 15 Birds' egg-albumins 8 



(b) -2 Reptile 



(c) 3 Amijliibian ,, 2 

 {(l) 6 Fish ,, G 



129 Bluod Sera 



Cln Chelonia 2 



4 Crococlilia 3 



l:^ Laceitilia 13 



28 Ophidia 2(i 



18 AmpLibia 17 



39 I'isee.s 3'J 



12 Arthiopoda 12 



60 Reptilia . 



This was the most powerful anti-cheloniaii serum made. Lar^'e 

 reactions occurred with 'S7 "/O <»f the Chelonian bloods tested, but slight 

 reactions only with a few other specimens of Re})tilian blood. 4() "/o ot 

 the Birds' egg-albumins tested gave some indications of clouding. 



