Graham-Smith 357 



This antiserum was only of moderate power, pnjducing- with its 

 homologous serum a medium clouding, followed by a well-marked pre- 

 cipitum. Only three doubtful cloudings were, however, obtained with 

 other fish sera, and other tests made with it were entirely negative. 

 Several tests were made with Limidus, and Arthropod sera, but with 

 negative results. 



V. Anti-Ascidian Serum. 



Tests with Anti-Ascidian Serum. 



This antiserum was obtained by the intraperitoneal injection into a 

 Guinea-pig of Ascidian extract. The extract was made by cutting large 

 fixed Ascidians into small pieces, and extracting these with salt solution. 

 The supernatant fluid which was used in these experiments was clear 

 and of a light yellow tint. 



Percentage 

 „ , of positive 



Material tested • « .' * x + + reactions 



14 Epg-Albtniiiiis 14 .... . 



22 Blood S,'m 



6 Amphibia 6 .* • . . . 



3 Pisces 3 .... . 



13 Arthropoda 13 .... . 



2 Ascidian extracts -.1.1. 100 



This antiserum produced a well-marked precipitum with its homo- 

 logous extract, and a slight reaction with an extract of a species of small 

 fixed Ascidian, but failed to give any trace of reaction with any of the 

 blood sera or egg-albumins. 



VI. Anti-Crustacean Sera. 



(1) Tests with Anti-Lobster Serum. 



This antiserum was made by Dr Nuttall by the injection of Lobster 

 {Homarus vulgaris) serum into Rabbits. 



Material tested . ^ ? ^ x 



14 Egy-Albuinina 



(a) 13 Birds' egg-albumins 13 . . • 



(b) 1 Reptile „ 1 . . . 



53 Blood Sera 



20 Reptilia IH 2 . . 



12 Amphibia 10 2 



12 Pisces 12 . . . 



9 Crustacea 2.1. 



