APPENDIX. 



Note 1 (to p. 10). Wright {Brit. Med. Journ., vol. i., p. 1069,. 9. v. 1903) 

 observed a decrease (" negative phase ") of the bactericidal power of blood serum 

 following inoculation with anti-typhoid vaccine, the antibody subsequently' increasing 

 in amount ("positive phase"). The haemolysins and anti-rennet behave in a 

 similar manner. 



Note 2 (to p. 112). Regarding Anti- Peptones: Dr E. F. Bashford has sent me 

 his Report to the Scientific Assessors of the Worshipful Company of Grocers, 

 London, dated 25. iii. 1902, in which he writes : 



" In his paper on immunisation against various proteids, the late Dr Myers 

 stated, that the precipitate produced by the reaction between Witte peptone and 

 the serum of an animal immvmised against it, did not give the biuret reaction, and 

 therefore was a new product. By the kindness of Professor Liebreich I was enabled 

 to immunise two goats against Witte peptone (albumose), and thereby to obtain the 

 product referred to in. large amount. It seemed that this step was likely to throw 

 much light on the nature of the reaction, and on that between toxine and antitoxine. 

 It was, however, found that the reason why the biuret reaction had failed was 

 simply the extreme insolubility of the product. Thoroughly dried in vacuo over 

 phosphoric anhydride along with a speciinen of the mother albumose {i.e. which had 

 been boiled and filtered as for injection into the immunised animals), both were 

 submitted to elementary analysis, with the following mean result for two analyses : — • 



Product precipitated — C4g.o Njg.jg Hg.g4 per cent. 

 Mother substance — C^j.jj Njg.e^ H^.g per cent. 



This did not afford grounds sufficient for speculation as to the nature of the 

 product precipitated, nor as to the mechanism of its production. I\Iany other 

 difficulties, especially the fact that albumoses in presence of other albuminous 

 bodies in neutral solution may yield precipitates made it doubtful if the product 

 analysed had any claim to be considered sufficiently free from admixtures, etc. 

 I have not given \ip this investigation, on the contrary hope that immunisation 

 against nucleohiston now proceeding at Dr T. H. Milroy's suggestion, may aSbrd 

 means for obtaining a reaction product more distinctly differing in composition 

 from the mother nucleohiston than was found to be the case for the albumoses of 

 Witte peptone." 



Note 3. On looking through the Index Jledicus, a paper by W. d'E. Emery 

 {Barfs Hosp. Joiirn., London, 1902 — 1903, x. pp. 34—40) relating to the precipitins 

 was noted, but too late to be considered in this volume. 



