28 GUrufli. and Sweet: 



ference of the lice, but showed no sio;n of nodules either in the brisket 

 or on the thigh, and no filarial parasites at all. 



Two cows, each containing a considerable number of nodules, were 

 imported to Melbourne from North Queensland, and placed in two 

 separate pens. They were examined very carefully at different times 

 on and after arrival for ectoparasites, with negative results. A locally- 

 bred 6-months-old calf was placed with each cow, each calf carrying 

 numbers of Tricltodectes scalaris (Haeniafopitvus vitiili and H. eury- 

 sternus not being then available), these being also found later on the 

 C'Ows. A large number of //. vlfuli and some //. eurysterrius. and a 

 large quantity of their eggs were later placed on each cow, especially 

 just over the nodule region. Large numbers of each kind of louse were 

 examined for larvae up to and about 5 weeks after they had been 

 placed on the cows, and at intervals later, but always with negative 

 results. That the worms in some at least of the nodules were living 

 was shown on arrival by excision of one of a large group lying under 

 the skin, living larvae and eggs being numerous. The blood of the 

 animal was examined during the operation, but no larvae could be 

 detected. Nor were any larvae or intraparasitic parasites found in the 

 sediment in saline fluid in which this living nodule had been kept at 

 blood heat for vsome time. 



Fluid aspirated after several blisterings in various ways, was 

 examined at different times after the oedeuux had been produced, both 

 on the same day and on the succeeding day, but in no case were larvae 

 to be found. 



Further numerous examinations were made of the blood at all hour.? 

 of the day and night for larvae, but none could be fnund even after 

 considerable quantities had been centrifuged. and a very large number 

 of smears, both thick and thin, examined in many ways. 



It is well to remember that embryos of Onchocerca volvulus, which 

 forms similar subcutaneous tumours in natives in West Africa, have 

 not yet been found in the blood of infected natives; as Bnunpt (p. 457) 

 very naturally suggests, they may in that case pass into the bloou 

 intermittently, or at certain times when the patient has not been 

 examined. 



As stated above, we (and we believe others also) have made exhaus- 

 tive examinations at all hours of the day and night. There is. how- 

 ever, one possil)i]ity Lingard (p. 2'2) has found in tlie blood of horses 

 in India, affected by Fihirin cquimi (?) and Fi/aria sp., that compara- 

 tively few embryos are present in the blood between July and Septeni- 

 ber, and may he even absent after that date. Thoy wore more 

 numerous between December and June, being most generally present 

 from April to Scjitember, during the hot and rainy seasons. This 

 supports the suggestion we have already iiuide to the effect that a sea- 

 sonal ])criodi(ity may be found in 0. t/i})soni. 



