C';-u6>' Inoculation of the Hoot Tubercle Bacteria. 197 



sowing'. iNieli infusion was diluted with tlic IxiiK-d watci-. and 

 purtiun of it used to w atei- the pots, the leniainder heing further 

 diluted and used for tliree subse(iuent waterings spread over four 

 or five days, so tliat in this way. the bacteria were iirHULrht into 

 intimate contact with the seeds and young roots. 



PoKS/hle )'('siiffs of Cross-J /loculftf ion . — Tlie imdules from the 

 various phmts sliowed irreat dilTereiice^ in size, form and cohiur, 

 each Rind being easy of recognition. If nodule growth on tlie 

 jdants followed inoculation, tlien it seemed feasilile that the size 

 and sliape of the niotlier nodule should be repro(luced in the nodules 

 formed n\n>u the roots of the inoculated host plant. 



By microscopic examination it was noticed that there were distinct 

 differences in sliape ami size between the l)acteria from the dift'erent 

 native legumes, and that the root ncxlule bacteria from native 

 plants, were much larger than those examined from the nodtdes of 

 (lover and hu-erne. • 



FIG. II.— DRAWINGS OF BACTERIA FROM NuDULES OF THREE 

 DIFFERENT NATIVE bELiUMINOSAE ( x 6Q(»). 



1. Phxiylohlvm nodule bacteria, Conyreyate in chains, and are buiall roinul 



Ijacteria. 



2. Acacia nodule bacteria. Curved rods ot varying length, rounded ends. 



Longer than FlutijlobiHiv 

 :3. liwaihsona nodule bacteria. Bacteria found singly; short and stumpy, 

 • but ot regular size. 



