370 



R. E. I'arry, 



TABLE ri. 



I'hysicdl Properties of Fractions. 



Teiiiperatiii' 



/X1.V 



^ Cineot. 



015° Content of 



Fraction. 



Whole Oil 



50.0 c.c. 



- 1.4657 



.900 



35.0 c.c. 



A table of this kind is extremely useful in pointing' out possible 

 constituents. For instance, fraction IV. is practically optically 

 inactive, and yet on determining its cineol content we have 

 accounted for only two-thirds of the fraction. This suggests the 

 presence of another optically inactive substance of about the same 

 boiling point as cineol. and further investigation confii'ms this. 



/(lent ificaf ion of Chief Const it ueiitx. 



Cineol. — As stated earlier, the camphoraceous odour of cineol is 

 noticeable in the crude oil. To confirm its presence, syrupy phos- 

 phoric acid was poured on to the oil in a test tube — equal quantities 

 of each — and the mixture shaken up and held under a running tap 

 fur a minute, when it solidified, proving the presence of cineol. 

 This test also proved the absence of aromadendrene, as the solid was 

 wliite. for, if it were present, a brownish-red coloration would 

 have been produced. For the quantitative estimation of cineol. the 

 resorcin method was used. The results obtained were : — In whole 

 oil. 72.2 per cent. In fractions. 70 per cent. A phosphoric acid 

 estimation was also carried out, giving slightly lower results, l)ut 

 not sufficiently diffei-ent to cast any doubt on the moi-e consistent 

 resoicin results. 



I'inene. — Fi-actioiis I.. II. and III. of the cleared oil was sepa- 

 rately decineolised by resorcin. The residues from fractions I. and 

 II. had distinct pinene odours, and were therefore tested for pinene 

 thus : 2 c.c. of the oil were cooled ; 7 c.c. t)f Amyl nitrite were added 

 and then 4 c.c. of glacial acetic acid. The mixture was then cooled 

 with ice and kept cooled while pfinal parts of glacial acetic acid and 

 concentrated hydrochloric aiid wen- added, drop by drop, with 

 stirring, as long as the licpiid was blue in colour. Crystals gradu- 



