294 Ethel McLennan : 



(9) The ovum is infected before any divisions have taken place- 

 in it. 



(lU) The hyphae aggregate at the proximal end of the develop- 

 ing grain. They are here used by the endospermic cells 

 in tlie embryonic pocket for food-supply for the develop- 

 ing embryo. 



(11) The association of the fungus with Lolhim temidentum 



and Lolium perenne. is probably a well-marked case of 

 symbiosis, comparable in many respects with that met 

 with in Callinia vulgaris. 



(12) It has been suggested that niti'ogen fixation was the function 



of the fungus, but an experiment has been performed, 

 and the result obtained showed that the fungus of 

 Lolium perenne is unable to fix nitrogen in the total 

 absence of external supplies of combined nitrogen. 



The foregoing worlc was carried out in the Botanical Depart- 

 ment of the Melbourne University during the years 1917, 1918, and 

 1919. I have to thank Professor Seward, Cambridge; Mr. S. F. 

 Armstrong, Agricultural School, Cambridge; Dr. Stoward, Western 

 Australia; Mr. Burtt Davey, South Africa; Mr. Breakwell, Sydney;. 

 Vilmorin-Andrieux and Cie, Paris; and the Royal Botanic Gardens, 

 Kew, for forwarding supplies of grain from different parts of the 

 world; also Mr. O'Brien, Assistant in the Botanical Department, 

 Melbourne University, for the help he has att'oided in assisting 

 with experiments and taking photographs. To Professor Ewart I 

 am indebted for the facilities provided for tlie work, and for much 

 helpful criticism during its progress. 



BIBLIOGRAPHY. 



( ) V«gl, A. E. — Zeitschrift flir Nahrungsmitteluntersuchung,. 



Hygiene und Vv^aarenkunde, Vol. XII., Jan., 1898. 

 ( M Guerin, P. — " Sur la presence d'un Champignon dans 



I'lvraie."— Journ. de Bot., Vol.. XII., Aug. 1898. 



(3) Hanausek, T. F. — " Vorlaufige Mitteilung tiber den von A. 



Vogel in der Frudit von Lolium temulentum entdeckten 

 Pilz."— Ber der Deut. Bot. Ges., Vol. XVI. 1898. 



(4) Nestler, A. — " l^ber e:n?n in der Frucht von Lolium temulen- 



•um, L en derkt-n Pilz." — Ber'. der Deut. Bot. Ges., Vol. 

 XVI. 1898. 



