296 Ethel McLennan: 



(21) Duggar, B. M.. and Davivs, A. R. — " Studies in the Physiology 



of the Fungi — I. Nitrogen Fixation." — Annals of the ^lis- 

 souri Botanical Gardens, Vol. III., 1916. 



(22) Schramm. J. R. — " A Contribution to Our Knowledge of 



the Relation of Certain Species of Grass Green Algae to 

 Elementary Nitrogen." — Annals of the Missouri Botanical. 

 Gardens, Vol. I., 1914. 

 (2:j) Koch, A.— "Handb. tech. Myk.," by Lafar, Vol. III. 



(24) Goddard. H. N. — " Can P'ungi Living in Agricultural Soil 



Assimilate Free Nitrogen?" — Botanical Gazette, Vol. LVI., 

 1913. 



(25) Ander.son, V. G. — " Tlie Detei'mination of Nitrites in Water."" 



— Soc. of Chemical Ind. of Victoria, Vol. XIII., 1913. 



(26) Freeman, E. Isl. — " The Affinitie»s of the Fungus of Lolium^ 



temulentum, L." — Annales Mycologici, Vol. IV., 1906. 



(27) Freeman, E. M. — " Symbiosis in the genus Lolium." — Minne- 



sota Botanical Studies, 19(34. 



(28) Hartley, IL, Campbell, N. H., and Poole, R. H.— " The Pre- 



paration of Conductivity Water." — Journ. of Chem. Soc.,. 

 Trans.. 19T)8. ' 



EXPLANATION OF PLATES. 



All figures have Ijeen drawn with the aid of the camera 

 lucida (Zeiss). 



Pl.\te XVIII. 



Figures 1-5 stained with aniline gentian violet, followed by 

 Gram's iodine wash. They illustrate the distribution of the fungus 

 in the grain, the position of the hyphae is indicated by the yellow 

 line shown in, the figures. 



Fig. 1. — A semi-diagramm,atic representation of a transverse sec- 

 tion through the distal part of a grain of Lolium 

 perenne. 



(a) aleurone layer; (b) cells of aleurone layer, which 

 stain differently fi'om the rest, probably ferment-contain- 

 ing cells; (e) starchy endosperm; (h) hyphal layer; (hi) 

 coloured portions of hyphae (the great bidk of the layer 

 is not stained with the gentian violet) ; (t) pericarp and 

 testa. 

 Fig. 2. — A transverse section of the proximal end of the same 

 grain. Letters as in Fig. 1, and (s) scutellum ; (el) epithe- 

 lial layer of scutellum. 



