150 



The sections were so badly disorganized in this last that they could 

 not be transferred to a slide. The observations were made on the 

 remnants. 



The castor bean is more resistive to the action of the cytase than is 

 the water lily. The tissues of the castor bean showed practically the 

 same effects as those of the water lily, but not quite so advanced. 



The endosperm of the wheat sections was more susceptible to the 

 action of the cytase than were the other sections. In the three days 

 germination extract, parts of the endosperm had dropped out, so the 

 sections could not be disturbed, while in the six days extract, only rem- 

 nants were left adhering to the aleurone layer. The aleurone layer and 

 the outer coats were unaffected. The middle lamella of the cells was 

 attacked first, as was shown by the cells separating whole from one 

 another. 



Sections were tested in the extracts from the flours, but were acted 

 on more slowly than those outlined, the white flour extract giving in nine 

 days, results equal to those obtained from the ungerminated extract in 

 three days. 



The sections were made from alcoholic material, so that there was no 

 protoplasmic action. Chloroform was used to prevent bacterial growth. 



Seeds which had germinated for varying numbers of days were sec- 

 tioned. In these, action was not so far advanced as in the sections placed 

 in the extracts. For instance, seeds germinated for six days, when 

 embedded in paraffin, and cut on the microtome, parts of the endosperm 

 still remained as a granular mass. 



