160 



On agar plates, the colonies are also about one-sixteentli of an inch 

 in diameter, but are somewhat irregular in outline, and very finelj' granu- 

 lar. 



On the agar streak, there is a luxuriant dirty-white, slimy growth, 

 giving a very slight fluorescence. 



On potato, there is at first a flesh-colored growth, later becoming a 

 dirty white, and on the very old cultures, a brown. 



Glucose solutions are not fermented. 



Nitrate solutions give a fair test for nitrites after 24 days. 



Milk is in no respect changed by this species. 

 SPECIES 4. 



Found in several nodules on Trifolium hybriduni. 



MORPHOLOGY. 



Bacilli occurring usually in pairs, rarely singly. 



In the nodules, these bacilli measure 1.5 mu in width, and 4 mu in 

 length. When taken from culture media they measure 1.75 mu in width 

 and 5 mu In length. 



BIOLOGICAL CHARACTERS. 



This form is a facultative anaerobe, non-liquefying, nou-chromogenic 

 bacillus, quite actively motile. Grows better at the body temperature than 

 at the room temperature. 



In gelatine stab cultures there is a scattered growth of individual col- 

 onies along the line of inoculation, without liquefaction of the gelatin. An 

 irregular button-like growth takes place on the surface of the gelatin. In 

 bouillon rendered slightly acid, no growth whatever took place, while in 

 neutral bouillon an abundant growth occurred. 



On agar streak a non-spreading flesh-colored growth appears, and on 

 potato a light lead colored growth follows the line of inoculation which 

 becomes slimy after four days. 



Glucose solutions are not fermented. 



Nitrate solutions are wholly reduced to nitrites. 



Milk is unchanged. 

 SPECIES 5. 



Found in nodules on several plants of Trifolium reflexum. 



