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the formalin solution, and, after a microscopic examination, were placed 

 in a moist chamber, the moist chamber being used in order to obviate 

 the necessity of using- vaseline to cement the cover slips to the glass cell. 

 Better results seemed to be obtauied when the culture had free access to 

 moist air. 



The data obtained from the formalin treatment which is given in 

 Table I, shows that the quarter-hour treatment in the weaker solutions 

 were apparently not effective. In the .18 per cent, solution with the quar- 

 ter-hour treatment every culture made showed good germination, while 

 in the .25 per cent, solution eight out of twelve showed germination in 

 the quarter-hour treatment and one out of twelve in the half-hour. 



TABLE I. 



Germination Tests of Spores Treated With Formalin. 



