304 iUinois State Labomtorij of NafuraJ History. 



cotton for the winter. Several of them were opened in the 

 spring and summer of 1884, at various dates, and found always 

 to contain only a pure culture of the original Bacillus, the 

 results of the first examination, made April 4, not differing in 

 this respect in any particular from the last, made July 30. 

 These bacteria stained much less freely than those in the fresh 

 culture, — a fact probably to be accounted for by their dormant 

 condition. Occasionally a spherical or subquadrate form, 1 f>. 

 to 1.25 /n, is distinguishable in the field by a deeper stain, — 

 possibly a spore of the preceding. 



Next came a culture in beef broth made by the usual method 

 from the contents of these tubes on the 23d of June, 1884. 

 Two days later this was slightly turbid, decidedly so on the 26th, 

 and on the 27th, when slides were made and the material was 

 used for an infection experiment, they were almost milky. The 

 contained bacteria now consisted of two forms: that frequently 

 mentioned above as Bacillus intrajjallens, and a spherical form 

 indistinguishable from rather large micrococci. The bacilli 

 occurred singly, doubly, and in strings, were 1 w by 3 ^ 

 in typical specimens, but varied considerably, especially in 

 transverse diameter, reaching sometimes a width of 1.5 i^. The 

 spherules, on the other hand, averaged about 1 ft in transverse 

 dimension. These occurred in various arrangement, but 

 especially in long chaplets. Many of them presented a slightly 

 quadrate outline and in a great number of instances strings of 

 these were continuous with shorter filaments of the bacilli. 

 Occasionally I satisfied myself that two or three of these 

 spherical forms were contained within the Bacillus cells; that 

 they were probably, indeed, to be considered as spores of the 

 cells or, as seems to me more consistent with the facts, as an 

 alternate form of the Bacillus. They seemed not to be devel- 

 oped by the transformation of the contents of an entire Bacillus 

 filament, but rather to be separated off from the end of such a 

 filament by a transformation of the protoplasm in the thickened 

 ends of the cells. 



Numerous other cultures were made from this same 

 material. One commenced July 30 was found, August 1, to be 

 decidedly turbid, and on the 2cl to have formed a thin trans- 

 parent pellicle over the whole surface. On the 3d this tube 



