25 



faneus, the small parasite living on Pliylloplwra Tlrodicci, 

 I was able to germinate some tetraspores of Choiulrus 

 crispus. The latter were dredged near Kiel, in the Baltic, 

 and sown in a sea-water culture in the Botanical Institute 

 of that University. 



Small portions of parchment paper were first thoroughly 

 soaked for a lengthy period, up to six hours, in running 

 water, so as to remove any acid present. The pieces of 

 parchment were of a size to he conveniently put on to a 

 glass slide, and covered M'ith a large coverslip for purposes 

 of microscopical investigation. These strips of paper were 

 put on to the bottom of small glass troughs 2" X 3" X 6" 

 being a convenient size. The troughs were filled with 

 fresh filtered sea water, and kept in a cool and fairly dark 

 place in the Laboratory. For the first two or three weeks 

 constant attention must be paid to the condition of the 

 water in the cultures. The water must be removed imme- 

 diately on the appearance of the slightest milkiness, the 

 outward sign of bacterial activity in connection with some 

 dead organism. A number of cultures should always be 

 set up, as some will always succumb to some adverse 

 circumstance. 



A portion of a fresh frond bearing a nemathecium may 

 be placed, as soon as obtained, on one of the strips of 

 parchment in a culture. After a certain time the spores 

 will be seen to have escaped, and to be lying about on the 

 parchment. The frond may now be removed, the spores 

 remaining in the culture. 



AYlien the spores begin to germinate the strips of parch- 

 ment can be put on to slides and be examined with the 

 microscope. They must be kept supplied with plenty of 

 fresh sea water, and be guarded against too strong light. 

 They may not be kept out of the cultures too long. A 

 coverslip may be employed, but with great caution. 



