228 



The activity of these cultures is prolonged by covering the medium with 

 sterile paraffin oil and chilling (0-10° Centigrade) just before their max- 

 imum growth (five to seven days) is reached. 



These cultures are pathogenic, altho, apparently their virulence is lowered 

 as indicated by a longer period of incubation and no fatalities. 



The cultural type differs from the normal Idood tyi^e of the spirochaete 

 morphologically and in its staining properties. 



This culture of Spirochaeta Novyi has been perpetuated upon ascitic 

 fluid containing undeli])rinated normal blood for six generations. 



REFERENCES. 



1. Deutsch. Med. Wchnschi.. 1909 XXXV, 835. 

 ibid., 1260. 



ibid., 1652. 



2. ibid., 1261. 



Klin. Jahr., 1910, XXiii, 339. 



3. Compt. rend. Soc. de biol., 1910 LXViii, 684. 



4. Ztschi. f. Hyg. u. Infeetionscbrankli., 1911 LXViii, 27. 



5. Deutsch. Med. Wchnschi., 1911 XXXVii, 682. 



6. Jour, of Exper. Med. 1911 XIV, 99. 



7. ibid., 1912, XV, 199. 



8. ibid., XV, 81. 



9. ibid., XV, 466. 



10. ibid. XVI. 194. 



11. ibid., XVI, 261. 



12. ibid., XVI, 620. 



13. Proc, Ind. Acad, of Sci. 1919. 223 



14. ibid. 1916 267 



15. ibid., 266 



