IOWA ACADEMY OF SCIENCES. 

 The following table shows the results of the work: 



97 



Q 



4) . 

 3 Ma 



z 



August 27 



September 15 



September 26 

 September 27 



September 28 



6|October 



540.000 

 1,076,000 



2,246,400 

 1,701,000 



1,200,000 

 3,510,000 



Fresh milk in sterilized flask, without preoautlons, from 



Oollege farm. 

 Skim milk from veterinary barn, brought from I. A. O. 



creamery. 

 Buttermilk, I. A. C. creamery. 

 Morning and evening milk brought to I. A. C. creamery. 



Six hours on road. 

 Milk from I. A. C. creamery. 

 College kitchen milk kept in room over night. 



Many of the species were cultivated and tests were made with several species 

 and different disinfectants. Corrosive sublimate, 1-1,000; pyoktanin, 1-1,000. 

 They were left in the solution for 30 seconds, 5 minutes and 10 minutes. The 

 material was taken up on the end of a platinum loop and placed in the disinfectant 

 solution with the following results: 



A COMMON BACILLUS. 



It was evident from the work that the material in direct contact with the dis- 

 infectant was destroyed, but thit in 'Aie interior of the ma?s was less readily acted 

 on, and grew, after a longer time, the disinfectant having not destroyed, but 

 merely inhibited the growth of the germ. 



Another experiment was tried in this case. The germs were thoroughly dis- 

 tributed in the solution and allowed to remain for a given length of time. A 

 platinum loop full of the material was added to a known quantity of agar, and 

 poured. The plates were allowed to stand twen.ty-four hours, with the following 

 results: 



CORROSIVE SUBLIMATE. 



1-1000 Ten minutes. Nogrowth. 



1-2000 Ten minutes. Nogrowth. 



1-3000 Ten minutes. Nogrowth. 



1-4000 Ten minutes. Nogrowth. 



PYOKTANIN. 



1-1000 Ten minutes. Nogrowth. 



1-2000 Ten minutes. Nogrowth. 



1-3000 Ten minutes. Nogrowth. 



1-4000 Ten minutes. Not all destroyed. 



