166 IOWA ACADEMY OF SCIENCES. 



and with G. macrojms from Cambridge, Mass., by Mr. B. M. 

 Duggar. All were complete failures. The spring and summer 

 were unusually dry. This probably accounts for the failures 

 with Fluke crab and wild crab. Natural cultures of BoesteUa 

 on wild crab were rare. 



In ihe spring of 1895 one of us being on Long Island, N. Y., 

 and the other at Ames, Iowa, we again undertook some experi- 

 ments with G. ■macropiis. We will speak first of the experi- 

 ments on Long Island. They were conducted in the nursery of 

 Isaac Hicks & Son at Westbury, N. Y. On May 18th, four 

 varieties were inoculated with New York G. macropus — Yellow 

 Transparent, Red Astrachan, Ben Davis and Red Pippin. The 

 first three were two-year-old nursery trees; the last was a large 

 tree. Many leaves on one tree of each variety were smeared, 

 both sides, with the gelatinous spore-masses of G. macropus. 

 The results were as follows: Yellow Transparent showed no 

 signs, whatever, of BoesteUa. Both Red Astrachan and Ben 

 Davis showed yellow spots which appeared like the beginning 

 of BoesteUa, but none of them developed. Red Pippin pro- 

 duced the BoesteUa, but the spores did not mature properly 

 and the fungus presented a stunted appearance. On May 24th, 

 six varieties were inoculated with Iowa G. macropus — Yellow 

 Transparent, Red Astrachan, Ben Davis, Red Pippin, Maiden's 

 Blush and Wealthy. All were two-year- old nursery trees 

 except the Red Pippin. One tree of each variety was inocu- 

 lated as before. The results were as follows: Yellow Trans- 

 parent and Red Pippin showed no signs of BoesteUa. Red 

 Astrachan and Ben Davis started BoesteUa spots which never 

 matured. Maiden's Blush and Wealthy developed numerous 

 BoesteUa spots and matured the aecidiospores thoroughly. As 

 no bags were used to cover the inoculated leaves, it can not be 

 said positively that the BoesteUa on Maiden's Blush and 

 Wealthy resulted from the Iowa G. macropus, but the condi- 

 tions were such as to warrant the above conclusions. In the 

 case of Red Pippin there can be no doubt as to which inocula- 

 tion produced the BoesteUa. A large tree which stood at con- 

 siderable distance from the other inoculated trees, was inocu- 

 lated on one side with New York G. macropus and on the other 

 side with Iowa G. macropus. The leaves of the branch inocu- 

 lated with New York G. macropus, and a few other leaves in 

 the immediate neighborhood, produced BoesteUa while the 

 remainder of the tree showed not a BoesteUa spot. It is also 



