IOWA ACADEMY OF SCIENCES. 



119 



WATER FREE SUBSTANCE. 



Sample 1 

 Sample 2 

 Sample 3 

 Sample 4 

 Sample 5 



25.56 

 34.84 

 41.47 

 37.55 

 33.33 



In the above results we find that the amount of water present 

 in the grass as received in the laboratory is very constant, the 

 highest being 78.96 per cent and the lowest 73.46 per cent, a 

 difference of only 5.5 per cent for a period of six weeks. In 

 the comparison of the results of the analyses based on the water 

 free substance in the fat content we find that the amount 

 decreases comparatively little as the grass matures. However, 

 with the amount of protein present there is a marked decrease 

 from 38.98 per cent to 14.46 per cent and in the albuminoids from 

 26.99 per cent to 8.83 per cent. The crude fiber increases on 

 the contrary from 16.23 per cent to 32.92 per cent. The amount 

 of nitrogen free extract present varies greatly. There is no 

 constant increase but it varies from 25.56 per cent to 41.47 per 

 cent. 



The constituents of an inorganic nature of ash remain quite 

 constant. The following analyses have been selected for com- 

 parison with the work of this laboratory: 



1. U.S. Dept Agricultural Experiment Station Record VI, No 



2. Bull. Iowa Experiment Station 11, pp. 432, 434. 



3. Bull. La. Agricultural Exparimeat Statioa 19, 2d series, pp. 538, 553. 



4. Ann. Report Agricultural Experiment Station Miss. 8, p. 92, 1895. 



5. Bull. Agricultural Experiment Station North Dakota, 15, 1894. 



6. Bull. Oregon Agricultural Experiment Station 39, 1895. 



p. 103, 1894. 



* Analyses are for natural or air-dry condition ; others are for water free substances 



