PREPARING TRICHINELLA SPIRALIS 301 



hain) were all tried. Iron hematoxylin seemed to give the best 

 results with borax carmine next. Where iron hematoxylin is 

 used, care must be taken to remove all the surplus mordant or 

 a precipitate will occur on addition of the hematoxylin thus 

 vitiating the results. The borax carmine has the advantage 

 in ease of handling. 



Clearing — Experiments with xylol, oil of bergamot, chloro- 

 form and turpentine showed that all these clearing agents shriv- 

 eled the specimens. As a matter of fact clearing is not at all 

 necessary. 



Mounting. — Specimens mounted in balsam were shriveled just 

 as when treated with a clearing agent so glycerine was used as 

 the mounting medium. The permanent mounts were ringed 

 with lacquer or thick balsam. 



It is, perhaps, needless to suggest that great caution be ob- 

 served in regard to cleaning cages in which hosts are kept, 

 means of disposition of their bodies and general cleanliness in 

 handling specimens. 



State University op Iowa. 



