NATIVE GRASSES AND PUCCINIA GRAMINIS 165 



According to Staknian and Piemeisel Puccinia graininis phlei-pra- 

 tensis are naturally infected as follows: Hosts on which P. gram- 

 inis phlei-pratcnsis was found in nature: Dactylis glomerata L., Fes- 

 tuca elatior L., F. pratcnsis Huds., Koeleria cristata (L) Pers., 

 Phlegm pratense L. 



Hosts heavily infected by artificial inoculation : Alopccurus geni- 

 culatus L., A. pratensis L., Holcus lanahts L. 



Weakly infected by artificial inoculation : Arena sativa L., A. 

 fatiia L., Arrhenatherum elatius (L) Beauv., Bromus tectorum L., 

 Elymus virginicus L., Hordciiui jubatitin L., H. vulgarc L., Lolium 

 italicum R.Br., L. perenne L., Secale cercale L. 



The writer and Miss King,^ in a paper on timothy rust report on 

 the unsuccessful inoculation of timothy rust. The work was started 

 in the fall of 1910; about fifty plants (several hundred leaves) of 

 timothy were inoculated by sprinkling upon them water containing 

 uredo spores of timothy rust. After a lapse of five days, uredo pus- 

 tules made their appearance. In addition a large number of experi- 

 ments were made with other grasses, foxtail (Sefaria glaiica), barn- 

 yard grass (Echinochloa crusgalli), blue grass (Poa pratensis), bar- 

 ley (Hordeum vulgare), brome grass (Bromus inermis), wheat 

 (Triticum vulgare) and red top (Agrostis alba). In none of these 

 cases was infection produced except in oats (Avena sativa). 



During the fall of 1911 inoculation experiments were carried on 

 with timothy, English rye, other grasses and oats grown in the 

 greenhouse. 



"Inoculations were as follows: on October 3, 1911, three pots of 

 young timothy plants, on the first leaf ; pot with 200 young plants, 

 inoculated with timothy rust ; pot with 500 young plants, with timothy 

 rust; pot with 200 young plants with red top rust. The method of 

 procedure was as follows : a quantity of spores, placed in about 2 1-2 

 cc. of water, was distributed over the plants ; the plants were then 

 protected by bell-jars for 48 hours. 



"On Oct. 9, no rust pustules were observed ; inoculations were 

 repeated upon the three pots of timothy plants. 



"On Oct. 9, young seedling plants of the following grasses were 

 inoculated with timothy rust ; wheat, oats, barley, English rye-grass, 

 Italian rye-grass, timothy, red top, meadow fescue. 



"On Oct. 18, rust pustules were observed as follows : on English 

 rye 2 sori, from timothy rust inoculation ; on timothy 2 sori from 

 timothy rust. 



^Timothy Rust: Bull. la. Agrl. -Exp. Sta. 131, 199-208, /. 1-4, 1912. 



