Twenty- THiBD Annual Meeting. 119 



444. Campyliurn chrysophyllum. Moist ground, dead wood, roots of trees, etc., 

 southeast Kansas. 



445. Campyliurn hispidulum Mitten. Ground, dead wood, etc., eastern Kansas. 

 (Rau.) 



446. Hypnum aduncum Hedw. var. Damp ground, central Kansas. ( Rau.) 



447. Hypnum curvifolium Hedw. Dead logs in shady woods, common. 



These 447 species of plants in Kansas, added to the 1,355 already published, 

 makes a total of 1,802 plants, not including hepaticfe, fungi, lichens, or algae. The 

 totals thus far are 1,666 flowering plants, 40 ferns and filicoid plants, and 96 mosses. 

 The largest order by far is the Compositge, embracing 292 species; next in size is 

 the grasses, comprising 204 species; and following that is the LeguminossB, con- 

 sisting of 128 species. Other orders, except the Cyperacese, are less than 100. The 

 future may reduce slightly, but will probably increase, these numbers. The work is 

 by no means ended. There is more to come. 



EXPERIMENTS IN 1890 FOR THE ARTIFICIAL DISSEMINATION OF 

 CONTAGIOUS DISEASES AMONG CHINCH-BUGS. 



BY F. H. SNOW, LAWRENCE. 



At the last meeting of this Academy, at Wichita, in October, 1889, the writer pre- 

 sented an account of his experiments in 1889 for the artificial introduction of con- 

 tagious diseases among chinch-bugs. These experiments have been continued from 

 the date of that meeting up to the prfesent time (November, 1890). 



Inasmuch as these maladies of the chinch-bug are not kept alive in the field 

 under ordinary out-of-door conditions of the winter season, the next important 

 point to be gained was their preservation through the winter in the laboratory, in 

 order that they might be under control and be available for use in the season of 

 1890. To accomplish this result, I placed fresh, healthy bugs in the infection jar 

 late in November, 1889, and was pleased to note that they contracted disease and 

 died in the same way as in the earlier part of the season. I was not able to obtain 

 fresh germs in the spring of 1890 until the month of April, and then only a limited 

 supply of live bags could be secured. I quote the following from my laboratory 

 notes: 



"April 10th, twenty-five chinch-bugs that had hibernated in the field were put in 

 the infection jars. They were supplied with young wheat plants. The bugs ap- 

 peared lively and healthy. 



"April 16th, some of the bugs were dead, and all appeared stupid. 



"April 20th, all of the bugs were dead. 



"One week later a new supply of fourteen bugs was put into the jar; they were 

 supplied with growing wheat. They ran substantially the same course as the first 

 twenty-five. Some had died at the end of the first week, and all were dead by the 

 end of the thirteenth day." 



The chinch-bug seemed to have been very generally exterminated in Kansas in 

 1889, and only three applications' for diseased bugs were received in 1890 up to the 

 middle of July. On account of the limited amount of infection material on hand, I 

 required each applicant to send me a box of live bugs, which I placed in the infec- 

 tion jars, returning in a few days a portion of the sick bugs to the sender. The 

 three applicants above noted reported the complete success of the experiments. I 

 —5 



