TWENTY-THIBD ANNUAL MEETING. 129 



development of bacteria in tubes charged with the filtered liquids after several days 

 is explained by the intentionally imperfect sealing (tubes 33 and 34.) The micro- 

 scopic examinations of the filtered liquids discovered no bacteria in the case of one 

 of the liquids, and a few non-motile apparently lifeless ones in the case of the other 

 (Mr. Stevens's examination). The presence of penicillium in the tubes is to be ex- 

 plained by anevident abundance of floating spores in the laboratory. These spores 

 must have been so abundant that in the short time the cotton plugs and covers were 

 removed from the tubes to allow inoculation, one of the tubes was infected (see 

 tube 4), and, more wonderful still, one of the flasks, while its tip was broken, man- 

 aged to receive one or more of the spores into its depths. (See turnip flask in 

 beaker 31.) It is possible that the spore or spores had fallen through the small mouth 

 of the filter and into the filtered, liquid, and thence been conveyed to the tube and 

 flask; or that the pipette used, though well flamed, may have been the agent of intro- 

 duction. The penicillium appearing ia tubes 9 and 14 was undoubtedly introduced 

 with the unfiltered turnip infusion, as this infusion had been more or less exposed 

 to the spore-laden atmosphere of the laboratory. However, the experimenters con- 

 gratulate themselves on the evident success of the sterilization of the culture tubes 

 and flasks. That the mould spores came from the atmosphere rather than from 

 the cotton used in sealing the tubes, is shown by the immediate appearance of 

 penicillium in those tubes left exposed. (See tubes 25, 26 and 27.) In addition to 

 the tests of the filter, observations on the effectiveness of certain methods of hand- 

 ling culture tubes were made, which need not be referred to here. 



FIRST ADDITION TO THE LIST OF KANSAS PERONOSPORACEAE, 



BY W. T. SWINGLE, B. SO., MANHATTAN, KANSAS. 



I published in vol. XI of these Transactions (pp. 63-87) "A List of the Kansas 

 Species of Peronosporacese" including the species found within the State up to 

 October, 1889. The paper was also reprinted with the original pagination, and dis- 

 tributed November 1, 1889. I have now a few corrections and additions to report 

 in the list. The additions to the list are arranged as in the original list, and include 

 all specimens collected from October, 1889, to November 1, 1890, and also a few col- 

 lected before this which were omitted from the original list. 



All new localities, host plants, or species new to the State, finding of oospores 

 on hosts where not previously reported, and collection of conidia or oospores in a 

 new month, are shown by the words indicating the fact being printed in BLACK- 

 FACED type. 



All of the numbers listed here are in my herbarium and also in that of Prof. W. 



A. Kellerman: 



CORRECTIONS IN THE ORIGINAL LIST. 



Page 64, line 6 from top, for Schlesems read Schlesiens. 



" " " 29 " " strike out and and add after Hedeomae and,P. Candida. 



" 66 " 20 " " for Leveille read Leveille. 



" " " 11 " bottom, strike out with immature oospores. 



" " " 4 " " " " ivith very young oospores. 



" 67 " 12 " top, after 1538 add with nearly mature oospores. 



" " •' 21 " " " on, add stems and. 



" " " 22 " " " 293 strike out ivith young oospores. 



" " " 24 " " " on, add stems and. 



