190 Kansas Academy of Science. 



of chloroform. The residues left on evaporating spontaneously the 

 etheral and chloroformic solutions were identical. Both residues 

 were examined carefully through a microscope for crystalline forms, 

 but none could be found. Both were of a yellowish-green amor- 

 phous substance, having a drug-like odor and a slight bitter taste. 

 The yield was small, 0.0979 grams being extracted from the 250 gm. 

 of millet. This residue or glucoside (?) was dissolved in 10 cc. of 

 alcohol, 5 cc. being set aside for future examination and the other 

 evaporated to 3.3 cc. at a temperature not above 55° C, and 3.3 cc. 

 each of water and pure glycerine were added to the 3.3 cc. alcoholic 

 solution of the glucoside, making 10 cc. of a 0.5 per cent solution 

 of the glucoside. 



This solution, on injecting it into the lymph sack of lively frogs 

 proved to have a decided toxic effect, even in very small quantities^ 

 At present we are not prepared to state definitely the quantity of 

 glucoside necessary to produce a fatal effect on frogs. 



Control experiments, using the solvent of the glucoside only, 

 demonstrated that the toxic effect was due to the glucoside present 

 in the above solution.^ 



CHEMICAL ANALYSIS OF HUNGARIAN MILLET. 



Air-dried Material. 



Crude protein. Fiber. Ash. 



6.7 % 31.6 % 0.23 % 



Oven-dried Material. 



Constant weight, 100° C. Seeds. Stems. Leaves. 



SiO. 18.73% 24.32% 22 62% 



F0O3AI.O3 4.15 4.62 3.85 



CaO 14.55 11.48 13 44 



MgO 2.78 1.82 2.85 



KNaS 58.84 55.89 56.12 



Totals 99.05% 98.13% 98.88% 



ANALYSIS OF SOIL ON WHICH MILLET WAS GROWN. 



Loss on ignition 11.04% 



Alkalies (calculated as potassium oxide) 0.45 



Acid insoluble 77. 10 



Ferric oxide 2. 56 



Alumina 6.34 



Phosphorus pentoxide 70 



Calcium oxide 83 



Manganese oxide Trace. 



Sulphur trioxide Trace. 



Magnesia .56 



Undetermined 42 



Total 100.00% 



From this examination it would appear that there was a toxic 

 principle of glucosidal character residing in this specimen of millet; 



1. For the study of physiolosical action I am indebted to Dr. Ida Hyde. 



