The Algx of Kansas Reservoirs. 



125 



In most cases the algae were present in sufficient quantities 

 to examine direct from the samples. In those cases in which 

 small masses of the algse could not be found in the basin or 

 reservoir a liter of water from the basin was taken and the 

 microorganisms present concentrated by the Sedgwick-Rafter 

 filter method. This method of determining the actual number of 

 microorganisms per cubic centimeter of water is especially 

 valuable in recording the reduction of organisms during the 

 copper- sulphate treatment. Most of the species found by this 

 method were diatoms and other unicellular algae. 



At times samples of living algae came into the laboratory in 

 a vegetative condition and the species could not be accurately 

 determined without the fruiting stage. This was especially 

 true of the Conjugatae. For such forms Klebs' method was 

 used to induce fruiting. According to this method, such forms 

 as Spirogyra, in a vegetative state, were placed in an aquarium 

 of about 2 percent Knop's solution and left to grow for a few 

 days. When a vigorous growth was shown the algae were trans- 

 planted to a jar of distilled water in the sunlight. In most 

 cases these conditions caused conjugation, aiding greatly in 

 classification to species. 



The key before mentioned contains an accurate and concise 

 description of the 110 species of algae found throughout the five 

 seasons covered by this survey, and a brief statement concern- 

 ing the locality of each. There are thirty-eight figures in this 

 report, drawn by the aid of the camera lucida from the material 

 examined, which will greatly aid the student of microscopy 

 in his identification and classification work. 



Below are listed the genera of the algae found throughout 

 this survey, and the number of times each was found : 



