286 Kansas Academy of Science. 



life periods, daily and total reproduction, proportion of wing- 

 less and winged forms, and of sexes, were also noted to a cer- 

 tain extent without interfering with the regular succession 

 of progeny. 



In attempting to conduct breeding tests to the fullest extent 

 and at the same time avoid too much complication in the pro- 

 cess, the establishment of an individual series became necessary. 

 As a matter of course, the stock for this series had to be drawn 

 from the generation series, but it was not allowed to dis- 

 arrange or risk in any way the direct line of descent nor to 

 lose its relative connection, else the entire work would have 

 been made worthless with regard to individual relations. 



Generation Series. An explanation of the method of con- 

 ducting the generation series is presented in the following 

 outline : 



For generation 1 establish series with a fertile female. 



For generation 2 isolate each of the young from mother of 

 series in consecutive order of daily birth, and number ac- 

 cordingly, 1, 2, 3, 4, 5, etc. Promptly designate each indi- 

 vidual as "Gen. 2, No. — ," for positive identification. Select 

 No. 1 as mother for generation 3 ; in case No. 1 fails of repro- 

 duction, substitute No. 2 ; or if this fails, take No. 3 ; and so on 

 by successive individuals if required further. Keep the life 

 records according to actual dates, but in reckoning the con- 

 tinuity of generations on the basis of development from first- 

 born individuals, deduct any loss of time, if more than a day, 

 which the substitute should have in place of No. 1. With 

 proper practice failures seldom occurred with more than two 

 or three consecutive individuals of a generation, and very 

 rarely amounted to as much as the loss of a day in difference 

 of time. 



For generation 4 and following ones, proceed in the same 

 manner, designating each individual upon isolation by the 

 proper "Gen. — and No. — ." 



Individual Series. The method pursued in conducting the 

 individual series requires the following explanation : 



This series should be begun with No. 2 of generation 2, 

 provided it does not fail and is not needed as a substitute in 

 the generation series ; but No. 3, 4 and 5 of the same genera- 

 tion should be held in reserve against possible failures or 

 accidents. All depends upon No. 1 having succeeded in pro- 



