Semi-Centennial Volume. 197 



Lot 33. Ground corn — defibrinated-blood protein nearly free from ash 

 -i- ash. Nutritive ratio 1:4. Ash 4 per cent of ration. 



Lot 34. Ground corn -^ starch 4- buttermilk casein -f ash. Nutritive 

 ration 1 :9. Ash 2.5 per cent of ration. 



Lot 35. Ground corn — buttermilk casein -i- ash. Nutritive ratio 1:3. 

 Ash 2.5 per cent of ration. 



Lot 36. Ground corn 4- buttermilk casein +ash. Nutritive ratio 1:6. 

 Ash 2.5 per cent of ration. 



Lot 37. Ground corn — buttermilk casein -r ash. Nutritive ratio widened 

 from month to month 1 :3-4-5-6-7-8. Ash 2.5 per cent of ration. 



Lot 38. Ground corn -f- egg-white protein nearly free from ash. Nutri- 

 tive ratio 1 :4. 



Lot 39. Ground corn -!- egg-white protein nearly free from ash -^ ash. 

 Nutritive ratio 1:4. 



The ash used consisted of calcium carbonate 92 parts, calcium lactate 

 8 parts, tertiary calcium phosphate 10 parts, secondary potassium phos- 

 phate 37 parts, sodium chloride 20 parts, sodium citrate 15 parts, and 

 ferric citrate 2 parts. Total 184. 



Distilled water was used instead of ordinary water. 



The purpose of the different lots may be briefly noted. Lot 30 was the 

 fundamental one, the pigs being given ground corn and distilled water 

 only. With this, lots 31, 32, 38, 33 and 39 are to be especially compared. 



Lot 31 received the same as lot 30, and in addition an artificial ash 

 consisting of a mixture of ash salts based on one used successfully by 

 Osborne and Mendel,* and which was very similar to Roehmann's suc- 

 cessful mixture. The change which we made was to omit magnesium 

 citrate, on the supposition that corn is adequately supplied with magne- 

 sium, and to supply a much larger amount of calcium in the form of 

 calcium carbonate. 



Lot 32 differed from lot 30 in that in addition to corn and distilled 

 water it received the proteins of defibrinated blood that had been treated 

 especially for the removal of salts. Lot 38 was similar to 32 except that 

 the proteins added were those of egg white freed form ash as nearly as 

 practicable. 



The pigs of lots 33 and 39 were given both ash and purified protein in 

 addition to the corn and distilled water. 



By comparison of the results of these six lots one learns the results of 

 feeding corn alone, and the effect of supplementing it by ash or protein or 

 both, and may observe the relative effect of proteins from two sources. 

 These proteins are complete in that they furnish all of the required 

 amino acid complexes. 



The nutritive ratio of corn is wider than is believed to be best for the 

 nutrition of young growing swine, that is, the carbohydrates are present 

 in too large an amount relatively to the protein, the nutritive ratio being 

 about 1 :9. The purpose of lot 34 was to ascertain how pigs would grow 

 on a ration of the same nutritive ratio as corn, 1:9, but which contained 

 casein, a protein that previous experiment had shown to supplement corn 

 perfectly. The casein added was balanced by corn starch. Ash was also 

 supplied this lot. 



* Pnblieation Ko. 156, Carne^e Institution of Washington, Part I. p. 32. 



