139 



13- Stain with Mayer's acidulated carmine, Mayer's haem- 

 alum, or otlier stain (p. 133). 



14. Wash out stain with 70^ alcohol, or water if aqueous 

 stain is used (p. 133). 



15. Remove to 95^ alcohol (p. 134). 



16. Differentiate with acidulated 95^ alcohol (p. 134). 



17. Remove to neutral 95^ alcohol (p, 134). 



18. Remove to absolute alcohol (p. 137). 



19. Clear sections with xylol, cedar-wood oil, or other 

 clearer (p. 137). 



20. Mount in balsam. 



21. Label. 



22. Harden balsam mounts in paraffin oven. 



FOR MATERIAL STAINED IN TOTO. 



Vary the preceding course of procedure as follows : 

 Between 2 and 3 stain in toto with alcoholic borax carmine. 



or other stain (p. 137) 



Omit 12 to 18 inclusive. (It is possible to mount directly 



after removing paraffin with xylol, but the mount is likely 



to be unclean unless fresh xylol is used for removal of the 



paraffin.) 



lU 



