RPIKOCH.ETOSIS OP SUDANESE FOWLS 
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used for inoculation, as well as the endoglobular forms. The point is a little difficult to decide, 
owing to the presence of so many immune birds, and this difficulty could only be obviated 
Ijy rearing a special lot of chickens and employing them in the work. I hope to carry out 
investigations in this direction. 
January 1.1st. Bird distinctly stronger and improved in condition, no doubt owing to 
care and good feeding ; but, on 
January loth an enormous infection was present, and in many corpuscles as many as 
five, six, or seven bodies were present. As indicated, this may not represent invasion by a 
corresponding number of spirochsetes. 
After this the infection seemed to diminish in severity, no doubt because some of the 
incorporated spirochetes had broken down and been discharged as tiny granules from the 
corpuscles. The blood was examined every day till 
February 7th, when a distinct increase in the number of bodies was apparent. 
February Dth. A great infection was visible. The bird was somnolent and prostrate. 
Death occurred, preceded by a marked fall of temperature and convulsions. 
Post mortem .—There was no gross lesion of any kind visible. Neither spleen nor liver 
was enlarged. There was some congestion of the renal vessels, and the kidneys had a mottled 
appearance. 
Smears were made from the heart’s blood, lungs, liver, spleen, kidneys, cerehro-spinal 
fluid and bone-marrow. The last named was dark red and fluid. In all, the bodies were 
present in the red cells. The lung smears were the most interesting. In these many 
corpuscles were seen in which the extra-nuclear cytoplasm had aj^parently vanished, l)ut where 
large, irregular forms of the bodies still remained, doubtless retained by an unstained envelope 
from which the haemoglobin had escaped. The substance of the bodies was broken up into 
little rods and dots which took on the chromatin stain. Some of these bodies were 4 p in 
either diameter. 
In this case and in two others I was able to study sections of the spleen, lung, liver and 
kidney. The tissues were fixed in perchloride of mercury and alcohol, embedded in paraffin, 
and stained with Leishman and eosin-haematoxylin. Beyond what appeared to be a kind 
of chronic venous congestion, I could detect nothing abnormal, save, perhaps, in the liver of 
Hen 20, where there was a marked leucocyte infiltration round the periphery of the lobules 
and extending into them between the cells. 
I compared these sections with those prepared from the organs of a healthy fowl. 
The next cases are of greater interest because the condition was followed through 
from the acute stage to the “ after phase,” and some interesting inoculation experiments were 
performed. 
Case II .—Foivl I .—December lind, 1907. Admitted suffering from spirochajtosis. 
Infection severe and bird distinctly ill. 
December 23rd. Spirochsetes numerous. Blood, about 5 c.c. in each case, taken froyi 
wing vein and inoculated into— 
(а) Fowl 33, which showed a large number of intra-corpuscular forms; 
(б) Fowl J, which showed intra-corpuscular forms in very small numbers. 
N.B .—In neither case was the inoculation successful. 
December 16th The crisis had occurred, and all the spirochsetes had vanished from 
the blood. About 5 c.c. were inoculated into— 
(i) Fowl K, a healthy young bird. 
N.B. - Eesult negative. 
From December loth to December 19th, the bird improved in health, ami the exiunina- 
tion of its peripheral blood was uniformly negative, both as regards free spirochsetes and 
