146 
KRPOKT OF TRAVELLING PATHOLOGIST AND PROTOZOOLOGIST 
Rosettes of 
Ucrpetovtonas 
Ilerpefomoncis 
in an 
Anopheline 
mosquito 
PJasinodia 
Typical free forms are shown at figs. 4, 6, 8, 9. The body is elongated and blunt at either end. 
The micronucleus is not far from the macronucleus, and there is a free flagellum. Never were 
true trypanosome forms seen nor forms with the microuucleiis on the non-flagellar side of the 
macronucleus. In some cases (figs. 2, 6, 10) one side of the parasite was evidently thinner 
than the other, and this had the appearance of an undulating membrane, with this difference, that 
the flagellum did not run along its margin, as it does in true trypanosomes. This membranous 
lateral extension of the body is especially well shown in fig. 6 and in some of the forms in 
fig. 10. It will be noticed that this extends the whole length of the parasite ; and if, for instance, 
in the form depicted at fig. 6, the flagellum were bent backwards and attached to this structure, 
the appearance of a true trypanosome would be produced. Whether this structure is truly 
homologous with the membrane of a trypanosome or not cannot be stated, but the appearance 
gives some support to this view. In the resting forms the body becomes contracted to 
approach the spherical and the flagellum shortened. Multiplication in this stage goes on 
producing the rosettes which have been described for other Herpetomonas. The individuals of 
these rosettes vary much in size (Plate XL, figs. 4,5, 7, 10). Each rosette appears to be the 
product of one Herpetomonas which has attached itself to the gut wall. As the rosettes increase 
in size, adjacent ones run into one another, till, finally, the whole gut wall is covered with 
a layer of these flagellates and the condition shown in Plate XI., fig. 15, is produced. 
The significance of these Herpetomonas, from the point of view of trypanosomiasis, has 
been discussed above. Several attempts were made to infect rats by inoculating them with 
a citrate emulsion of infected gut of Tabanus socius, but with no result. 
M Y z o M YI .v N I L I 
Plate XI., figs. 11-14 
Though some hundreds of mosquitoes of different kinds were dissected at one place and 
another, in only one species w'ere flagellates found. This was in one Myzomyia nili at Nasser, 
on the Sobat, in wfliich a species of Herpetomonas was discovered. These differed from the 
forms found in the Tabanidce at the same place in that the non-flagellar end of the body w'as 
drawn out into a fine pointed extremity. In some there was the appearance of a short 
membrane (fig. 1-3). Eesting forms occurred as well as free forms, and these were either pear- 
shaped or spherical. None of the forms met with showed the true trypanosome structure. 
Gloss IN.4 P.4li>alis 
At Wau and on the Sueh river, about ten miles above Wau, twelve Glossina palpalis 
were dissected, but no flagellates were present in the guts of these. The number of flies 
dissected was too small to allow of any conclusions being drawm. 
On several occasions Hippoboscidee and Stomoxys, taken from domestic animals infected 
with trypanosomes, revealed these parasites in their intestines, but there was nothing to show 
that their presence there was anything more than accidental. 
Plasmodia 
Plasmodium mabuim, n. sp. 
Host, Mahuia qiiinqueUeniata. Locality, Wau, Bahr-El-Ghazal Province 
Plate XII., figs. 1-12, 14 
This parasite was discovered in the red corpuscles of a lizard {Mahuia qmnqueUeniala) 
which was very common in and around Wau, where it was seen either in the grass oi' on the 
