106 



A H.KMOnHF.r.AItlNE OF MAMMALS 



Mcasurt'nienls 

 of vcmiiculcs 

 and flagellates 



Kic. 69.— CouoslB KAUlttL (x 2000 diam.) 



Possible 

 explanations 

 of appearances 

 observed 



(</) Kliigfllatrs. 'I'lifsi' luxlifs wciv <ir 11 (listiiicl (r\ |PMii(>s(iiiic or tr\ |i:iiii>|ilasiiia I y|ir. 

 Fliigfllit cillicr at oiic or both imhIs, wen- clrarly visible, ami tlicy wcrr in a state of very 

 lU'tivc viliratile nuitioii, tlioiigli tliev ili<l not seem to possess mneh motion of translation. 

 (Plate XIII., Fi- /..) 



All the forms were waUlncI for several liours 

 amino clinngcs were noted, save that some of the 

 VcriuietlleS heealne motionless anil others a|i]ieareil 

 to change into typical llagellatcti forms. 



Stained preparations were seoireil of all these 

 forms, and a rosette of vermiciiles is shown in 

 Pliite XIII., Fig. '/, and in Fig. 50. Tluse were 

 ohtaineil from a Ilea, dissected si.xteeii hours after 

 feeding, in which sjiherical forms and vermiciiles, 

 either free or in small rosettes, were the only forms 

 foMiid. It will be noted that the blephuroplasts are 

 large and are towards the centre of the rosette, /.(., 

 towards what are probably the anterior extremities 

 of the verinieiiles. 



These veniiicules measured 75^, to 9^, in length. 



The stained forms from Flea 1, Plate XIII., Fig. r, gave the following measurements: 

 Vir III hulls — 75 /I, '.I /I, and \'l /i in length. 



If) /I, to '1 n iu breadth at nuclei. 

 /'A»</.//rt/<.s— Total length .. .. IdS ,. to I 15 ^. 



Length of Hagella . . . . :'. /i to 45 ,i. 



Some ol' I lie trvpaiioplasma forms were found to contain two small spherical chromatin 

 masses in addilinn to the nucleus cu- karyosome. These may represent the " diplosome " of 

 Prowazek.* (Plate Xlll., Fig. c) In neither the vcriiii<iilcs nor the tlagcdlated forms was 

 there any indication of an undulating lueiiiliiaue. 



The spherical forms po.ssessed well-marked uu<lei. and there were also seen what 

 appeiired to be intermediate forms between them and the vermiciiles in which blc[iliaroplasts 

 had develoi)ed. (Plate XIII., Fig. '.) 



[Ordinary iinchangeil tro}ihozoites of the Ifn iiiiujnijnrini' and a few large vermieules 

 like those found in the peripheral blooil of the jerboa were also met with in the .stained 

 preparation. They had not been observed iu the fresh disseetiou.] 



It seemed to me that these appearances might represent one of three things: 



1. A special parasite of the Ilea of the nature of /fi r/iiti)Wiiiiiis or ('ri//iii/iii. 



•I. A development of trypaiiosomes in the Ilea which, though taken from a jerboa, 

 might possibly have fed on a gerbil with trypanosomiasis, but vide "3." 



X A cycle of development of the luemogregarine of jerboas, somewhat aiialagous to that 

 de.seribed bv Schaiidinii for Hulti riilnim i/miiliirdi/i in Ci'/'.r jiijii'ii-'i. That the last was the 

 correct explanation I at Hrst thought i)robable. Supposition 2 was put wholly out of 

 account, as several fleas in which these appearances were found could not have fed on a 

 trypanosome-iufected animal, while in one flea dis.sectetl twenty-four hours after removal 

 from its host, I observed in the posterior part of the mid-gut auiceboid-looking forms, some 



* Arlj. aii.s d. Kuis. QchundbuiUamtc XX., 1904. 



