1-20 TllYl'ANOSOMlABIH IN TIIK ANGLO-EQYl'TIAN SUDAN 



111' prooi'iMls tu jKiiiit imt liuw ililV.iviit in morphology is this tryimnosonu' fmin 

 T. /Iriirri iiiul T. h'nni.ti. He foiupiires it with T. Thnln-i, the giiiiit trypuiiosonie of South 

 Africiiu cuttle, and coiuludes by reinurkinf^ thiit while very distinct from T. Tlniliri, 

 T. ittiiiiim iiiiproiiilies it in being peculiar tu cattle, so far as is at present known. 



I have little to add to the above description, {riifr Piute XIV., Fig. >i.) 



'I'll.- photo-micrograph (Fig. 65), for which I am indebted to Dr. Beam, chemist to the 

 Laboratories, gives a very fair ideu of one of the shortest forms of 7'. luiintm. It shows it 

 to be u short trypanosome with hardly uny free ilagelluni visible, but is not (juite typical in 

 that the po.sterior moiety is rather broa<ler than is usually seen. 



I append measuremeuts I have made of a form whose total length was 14 /i. 



From posterior cud of body to centre of ccutrosomc I - /' 



From ooutre of ceutrosomc to nucleus I-/' 



Nucleust I !■ >« 



From iiudeuH to begiuuiug of Uiigellum .'>(;/i 



Free llngellum 1 I /i 



Breadth Ijcliiud micleus l! /i 



I agree tluit tlic pnitoplasiri is homogeneous, though it sometimes stains irregulurly, as 

 evidenced in Fig. (35, while in forms kept /// rltru granules appear, for the most part 

 anterior to the nucleus. In such forms tlic Viicuole in the neiglilioiuliood of the 

 ccutrosomc may be found large and very evident. Sometimes a portion of tlu' free edge of 

 the undulating membrane is clearly visible, liuiKlird as it were upon llie back of the 

 trypanosome and looking like a loop. As a nilc. howivcr, tlie undulating membrane can 

 scarcely be seen save in the living j)arasite. I havi- workeil witii specimens stained by the 

 Leishman-Komanowsky method, which answers admirably if the stain be strong and staining 

 prolonged. After fixing with the alcoholic stain in the usual way I am in the habit of 

 ailding an equal (juantity of distilled water and allowing the stain to act for from twenty 

 minutes to half-an-hour or even longer. 



I have carried out a few experiments in ri/ro wliich may be mentioned here, tiiough 

 the study of the trypanosome is yet far from complete, owing to lack of material and press 

 of other work. Hence cultivation experiments have not been attempted. 



In citrated blood kept at a temperature of 22^ to 23' C, no change in the trypanosomes 

 was vi.sible after twenty-four hours. They remained lively and stained well. After seventy- 

 two hours at a temperature of 25° C, changes were observed to have occurred, the posterior 

 en<ls of the parasites having become swuiKn, whiii' the organisms were sluggish und 

 evidently degenerating. 



Trypanosomes disappeared in 21 iiours from sterile citrated blood which had been 

 exposed to a temperature of Ki' ('. 



The trypanosomes from tin' Mrliit cow remained alive in non-sterile citrated blood at a 

 temperature of about 35 C. for twenty-four hours. They unilerweiit longitudinal division, 

 forms with two centrosomes and two nuclei being seen. In these the unilulating membrane 

 was more apparent than usual. 

 Inoculation Iiioriiliitloii K.ij)frliii(iits. From <>.i- \ii. 1. — 0'5 c.c. citratrd lijoiid, i.e. about 0'25 c.c. 



exiwriniunis l,),,,,,]^ was inoculated subcntaiieously into a moidvey {Cirri'pitliniis .v((/i/M(\) on ."lOlIi October, 

 I'.KM. 



On the same date a rabbit received 1 c.c. of citrated blood. These animals never 

 showed any symptoms of the disease, und though their bloods were repeatedly centrifuged 



