I'il TllVl'ANOKOMIAHlS IN THK ANCiLO-KO Yl'TIAN Sl'DAN 



" II. Laij^f forms iiii'iisiiiiiig 21 tu '.iO fi in liiigtli by 2/i in brcinltli. Thusc fdiiiis, 



ill which tlif ihigilluiii ixliihits a vi-ry long irw. portion, betir u great resuniblanee to 



T. Jimiisi. Thv postt'rior uxtrumity is usually tlongattd, tlic protojilasiii is homogenous 



anil with but fiw granules. Forms uiulergoiug division by separation into two elements 



are lounil. 



" It is to be noted that intermediate forms between the small and the largo 



trypanosonies are wanting." 



From a study of numerous preparations both fresh and stained I am in a position 

 to add a few additional notes to the above. 



Ill fresh blood botii forms of trypanosonies ean be elearly made out. The long forms 

 arc iiuirli tlif inure aetive, darting rapidly bitlur and thither, lashiug vigorously witli tluir 

 llagella, and disphieing the red blood eorpuseles. They ean advance with either tlie anterior 

 or posterior end in front, tiiough their lunger excursions are mailc with the flagelhini "going 

 on before." 



Occasionally one of these long aetive forms may be seen to traverse the field of the 

 microscope, but this is not euiiinion. The body of the trypanosome freipiently bends upon 

 it.-<elf so that it {iresents the a}>pearanee of a tiny corkscrew for the fraction of a second, and 

 then, stretching out, the parasite shoots across some space amongst the corpuscles, and 

 plunges writhing and lashing amongst a group of startled erythrocytes. 



In tlir fresh state the undulating membrane is not very well defined in these long forms. 

 They do not j)resent a granular aspect. The short forms on the otiier hand are, as a rule, 

 ilistinctly granular and are much more sluggish in tluir movements. They tend to hang 

 about the same spot and their excursions are limited, rather re.seiiibiing those of 7'. niiiiiim. 

 They also can advance witli either end in front, but tluir body nioveiiiciits are more of a 

 rippling or undulating type. It often looks us tliougli a series of shivers was riiiining along 

 the protoplasm. 



Their undulating iiieiiibranes are well marked, and llie rounded posterior ends are very 

 distinct. On staining with Leisliman-liomanowsky, used strong and for a considerable time 

 as in the ease of 7! uaniim, the differences in structure between the two forms are well 

 emphasized. Points to which Laveran in his short note does not refer, are the well-known 

 " pike-head " form of the posterior end of a typical long trypanosome, and the fact that the 

 centrosonie of tlii' long form is not as large as that of the small. 



In some of the short forms the nucleus seems almost to touch the centrosonie, while 

 " biiiudiing" of the undulating membrane is often well seen. I have noted curious forms, 

 jiossibly distorted, with stjuare-cut posterior ends, and more than once have seen a short 

 form witli no granules visible. 



As a rule the granules are in the posterior moiety, i.e., between the nucleus and tlie 

 centrosonie. In some iustances the possession of these chromatin granules is almost the 

 only point enabling one to distinguish this trypanosome from T. naxum. I have also noticed 

 ilividing forms, and it is not uncommon to find two short forms lying with their posterior 

 ends in close contact — possibly a pndiminarv stage to conjugation, more likely the terininal 

 stage of a division. 



In the mule's blood T did not observe conjugating or agglnfiiiating. or involution forms. 



1 agree that the long foiin< asure from '21 to 3U fi, but some are as narrow us r4 fi ut 



their thickest portion. 



