TRYFANOSOMIASIS IN THE ANGLO-EGYPTIAN SUDAN 14:1 



liver was congested, but the spleen looked normal. The mesenteric glands were enlarged and 

 one of them was hsemorrhagic. Bone-marrow, pale. Lungs, anaemic and retracted. Smears 

 were made from the heart's blood, liver, spleen, intestines, bone-marrow, glands and cerebro- 

 spinal fluid. In the last mentioned only were trypanosomes found — a few unaltered, 

 apparently long forms, being present, but they did not present a characteristic appearance. 

 In the heart's blood myelocytes and eosinophile myelocytes were present. 



Exp. 16. Gerbil 8. March 18th. Inoculated intraperitomally with a few drops of 

 blood from Dog 4. 



Blood examined daily and found to be infected on Marcli 22nd. Incubation period, 

 31- days. 



Trypanosomes of both forms fairly numerous. No " tadpole " fuvnis, like those described 

 in rats by Button and Todil, found. Punctate basophilic degeneration of the red cells was 

 present. It is connnon in gerbils, as is 

 polychromatophilia. '^^^ 



Trypanosomes were constantly t^^ ^"^ 



present in the blood in fair numbers 

 till death occurred on April 3rd. The 

 symptoms were drowsiness and pro- 

 gressive emaciation, no cedema, April ./-^^ m^i ^ ft ■ O 

 ird. Animal found dead in the 



p^' v%. J^ 



morning. O' Q »^ V ^ 0^\ 



Fost-movtem . — A bunch of nema- 



xs^ 





todes found in the stomach. That ^ ' (f * O ^b © 



organ contained altered blood and T O ^^* k^ C3 



thick, tenacious mucus. There were ~ '"^ 



congested points on the surface of the 



mucous membrane which looked as 



though they might have gone on to 



ulceration. 



Smears were made from these p,^ 72.-Forms seen in Blood. Monkev 4 (x 833 diam.). 



small areas. In these no spirilla 

 were found, but some very curious forms of trypanosomes were present. 



Thus there was a pear-shaped form, the bulbous part almost wholly surrounded by the 

 looped free edge of an undulating inembrane which sprang from a centrosome situated close 

 to the root of the long flagellum. The protoplasm was slightly granular, and there was a 

 distinct vacuoloid area beside the centrosome. Another quaint form was fish-shaped, with 

 a thick, short, blunt anterior extremity, a well-marked centrosome, vacuoloid area and a 

 nucleus which had lost its differential staining, and was indefinitely marked. The whole 

 parasite stained in a peculiar manner and was probably an involution form. 



In addition to these, tiny forms shaped like trypanosomes were seen. I am doubtful if 

 these are parasites at all. 



Their protoplasm stained a faint violet-pink or ruddy hue, quite a peculiar colour 

 while the centrosomes and nuclei were stained deep Ilomanowsky red. They possessed no 

 visible flagella and were fairly numerous. Plate XIV. ; Fig. ,/'. 



Faintly staining forms, with their posterior ends in contact, were also seen. 



Heart. — Ordinary trypanosomes, both forms present. 



