TBYPANOSOMIASIS IN THE ANGLO-EGYPTIAN SUDAN 143 



Lung, Bone-marroiv and Liver also showed altered trypanosorae forms. 



Spleen <tiid Brain. — No trypanosomes preseut. 



Exp. 20. (Terbil 10. — Weight 34 grams. This gerbil's bhjoil contained some red 

 cells infected in the manner to be described, 



April i/Ji. Inoculated subeiitaneously with •2.5 c.c. blood from Jlonkey 4. 



AjJril 8f/i. Trypanosomes, mostly long forms, found in the blood. Some short forms 

 showing granules were also present. 



Ap?il 8th. Treatment with chrysoidine begun [vide p. 157). 



Exp. 21. Monkey 5. {Cercopithecus sabwus). 



April lOfli. Inoculated with 1 c.c. blood from Eabbit 2 (Exp. 12). 



April 15f/i. Blood examined. Nil. 



April Vltli. Trypanosomes present. One or two per field. Mostly long forms. 



Ap7'il 22nd. Many parasites seen. Dividing forms noticed. 



April 24:th. Conditions the same as on April 22nd. 



Mai/ 8th. The animal has been getting thin. Mucous membranes somewhat anaemic 

 but blood of a good colour. Four or five trypanosomes seen in most fields. Faeces 

 examined microscopically. No spirilla or trypanosomes found. A few red cells and a good 

 many leucocytes present. 



Ma)/ 15//i. Monkey very ill and evidently nearing its end. Appetite still preserved 

 Trypanosomes not so numerous in the blood as formerly. Some apparently degenerated 

 forms showing vacuolation. Chloroformed at 3.15 p.m. and autopsy performed at once. 



Body emaciated. No scrotal oedema or eye infection. Mucous membranes markedly 

 anaemic . 



Heart.t blood. — Both forms present. The long trypanosomes extremely active, rapidly 

 traversing the field, and moving almost like spirochaetes. Nothing special noted in stained 

 preparations. 



Splcin. — Slight enlargement only. Malpighian bodies not visible on the surface, but 

 seem to be enlarged when section made. Some congestion present. On staining a smear 

 no vestige of a trypanosome could be found, a somewhat remarkable fact. 



Liver. — Slightly congested. Both forms found in smear. 



Stomach. — Vessels from smaller curvature engorged. Two areas of congestion shewing 

 early ulceration. But little necrosis present. A smear was made from one of these surfaces 

 but neither trypanosomes nor spirilla were present. 



Intestines. — Congestion and ulceration of Beyer's patches. In smears made from these 

 areas a few spirilla were found, similar in appearance to those discovered in the stomach 

 ulcer of Dog 4 (Exp. 9). 



C'cecHin. — A large punched-out ulcer was present, its surface covered with blood clot. 

 Smears made from the clot and from the ulcerated surface shewed neither trypanosomes 

 nor spirilla. 



Large Intestine. — Ulcerated areas present in the transverse and descending colon, 

 These ulcers are more advanced than those in the small intestine. One ulcer measured 

 \ inch in leiigtli and was markedly hajmorrhagic. A smear showed many bacilli, red and 

 white blood corpuscles and a vast number of very thin, lightly staining vibrios ami spirilla. 

 Some of the latter were of considerable length possessing from four to six undulations, but 

 they did not resemble the spirilla found in the ease of Dog 4 or those present in the smear 

 made from the small intestine. 



