m TKYPAN080MIA8I8 IN THE ANOLO-EOYPTIAN 8UDAN 



Fcrrrii. — These were soft, yellow iiiul not offensive. 



Afesi-ntrrir ijliimh. — Tllese were eliliirgeil ami yellow (fiitty ?). TIley were neither 

 congested nor hicmorrhiigie. 



Liiiii/n. — Jliirkeclly antemie. 



Kitliu'i/ii. — Tiiere iippeareil to he a very early lupiiritis. 'i'lieir capsules stripped easily. 



Jiitiif iiiiirroir. — Red iind rather Huid in eonsistenee. Simar showed no parasites. 



Bniiii. — ^llather ana-niic. A slij^lit increase of the eereliro-sjjinal Hiiid wliicdi contained 

 trvpanosonies. A stained smear of the tiuid showed nothin^^ Imt long forms. Some of the 

 parasites liad completely disintegratnl, |iiissilily as a result of tin- spreading out on the slide. 

 Only the centrosomes ami free edges of tli<' undulating nieniliranes, the latter prolonged into 

 the flagella, renniined. 



Some experiments /;/ vitni were condueitij uilli the trvpanosonies derived from the 

 heart's blooil of this monkey, as it was thought that a means of telling whether we were 

 dealing with only one or with two species of parasite, might thus be foun<l. Equal parts 

 of infected hlood and sterile citrate solution were placecl in sterilised glass tubes and 

 incubated at ST'^C, and at a temperature varying between 21()^C. and •24°C. respectively. 



.\ftcr '1\ hours a few somewhat sluggish trypanosomes were demonstrable in the warm 

 tube. In the cold tube lively forms were present. 



After 48 hours, living forms were found in both tubes, but those in the cold tube were 

 much more lively and also more numerous. Dividing forms were present in both tubes. In 

 the cold tube, baby try[)anosoiues were seen separating off from the panut parasites in a 

 manner resembling that fignreil in Laveraii and Mesnil's Treatise, Fig. XX., p. 211. 1 

 also noticed appearances suggesting those shown in Fig. II., p. 888 of the Journal of the 

 K.A.jr.C. for JIarch, 19U5, in connection with tiic cultivation of the Leishnian body. In 

 other words, slender siiirilla looking forms could be seen separating from the tiagellatcil 

 parasites. In stained preparations only the long forms were clearly recognisable, though 

 what might have been short forms were seen. It was very difficult to be certain. A general 

 appro.\imation to spirillary form was evi<lent. lioth ends of the parasites were pointeil and 

 the chromatin was somewhat diffused. Nuclei and centrosomes were apparent, but the 

 staining reactions lial altered, and in the specimen kept at 37"C., differential staining hiid 

 been lost to some extent. The flagella stained indifferently well. 



After 70 hours no trypanosomes were found in the tube kept at 87'-'C. In the cold 

 tube a considerable number of lively forms were ju'esent. A few motionless parasites were 

 also noteil. The trvpanosonies approached still niore closely to tln' spiiillar type, being 

 very attenuated. A ru<ldv staining of the cytoplasm was also noticeable. 



After !)2 hours the same condition of things was found. There was a still greater 

 approximation to the spirillar tvpe but no true spirilla were found. On staining, no flagella 

 were visible. Curiously altered forms were also present. 



After 110 hours it was found that bacterial invasion, always a difKcidt factor to 

 exclude in Khartoum, had taken place. No living parasites were found, but degenerated 

 forma could be seen. 



Exj). 22. fierbil 12. Weight 15 grams. This was a small dark gerbil, apparently a 

 different species from those hitherto employed. Its blood did not exhibit basophilia. 



A/iril H)l/i. Inoculateil with a few drops of blood from (Jerbil 10. 



Ajiiil Vlth. One long trypanosome found. Very lively. No " tadpole " forms seen as 

 described in T. dimorphum in rats by l)utton and Todil. 



