TRYPANOSOMIASIS IN THE ANGLO-EGYPTIAN SUDAN 145 



April 15///. Blood swarming. Treatment with so-called solnble chrysoidine begnn 

 and case therefore considered under treatment {vide p. 157). 



Exp. 23. — Rat (Mas decumanus). Blood free of parasites. 



Aji/il I'ltli. Inoculated subcutaneously with "25 c.c. blood from Gerbil 10 to see if 

 " tadpole ■■ forms developed. The blood was examined daily after April 14th till April •22nd, 

 but no trypanosomes were found. Accordingly Exp. 23a was performed, the rat being again 

 inoculated, this time from Monkey 5. 



April 23rd. Rat found dead. Spleen enlarged. Bacterial invasion of the tissues had 

 occurred and no parasites were found in smears made from the heart's blood and various 

 organs. 



Exp. 24. Jerboa 6. — This animal was used for an immunisation experiment with blood 

 serum and its case is accordingly considered under treatment (p. 170). 



Exp. 25. Monkey fi. — {Cercopifhecvs sahcrus.) Weight 1'3 kilos. 



April 24//t. Inoculated with 1 c.c. blood from Monkey 5. 



April 29tli. Trypanosomes present in peripheral blood. Nearly all long forms. 



May 5fh. Both forms present and lively. Twenty to thirty in each field. On 

 staining, the short forms were found to predominate. Many of them were very broad and 

 exhibited chromatin granules, vacuoles and large undulating membranes. Some of the long 

 forms were dividing. This monkey was treated by blood serum injections and its history is 

 therefore considered later (p. 1(38). 



Exp. 26. Gerbil 13.— May 5th. Inoculated from Monkey 5. 



Ma>/ St/i. Both forms present in tjie blood, the long forms predominating. 



Mill/ lOf/i. Animal funiul ilead and decomposing. Bacterial invasion of tissues. 



Exp. 27. Gerbil 14. — May 4th. Inoculated from Monkey 5. 



Map 8t/i. Both forms present in the blood, the long forms preponderating. 



Afau 21.'<f. Animal chloroformed and blood-agar tubes inoculated from the heart's 

 blood, in wliieli both forms were numerous, and incubated at 22° C. All the tubes became 

 contaminated. 



Stomach and intestines examined but no congestion or ulceration found. 



Exp. 28. Gerbil IG. — May 11th. Inoculated with a few drops of citrated blood 

 from Monkey 5. 



Mai/ 16///. Trypanosomes present in the blood but few in number. 



Maif 20///. Many fields devoid of parasites. Two, the greatest number seen in 

 one field. 



This gerbil was treated wth blood serum and so it is considered fully later (p. 169). 



Exp. 29. Gerbil 17. — J/"// 11///. Inoculated with a few drops of citrated blood 

 from Monkey 5. 



Ma>/ 16///. Trypanosomes present and numerous. 



AIay2Qth. Trypanosomes present and numerous, 6 to 12 per field. 'A stained film 

 exhibited both forms and, in addition, strange short forms dotted all over with chromatin 

 spots. There were also curious amorphous masses, showing vestiges of their having once been 

 trypanosomes. These apparently were the result of a natural degeneration. Those mth 

 the abundance of chromatin possibly exemplified an effort to cope with this destruction, 

 This was thought to be a suitable case for treatment by solnble chrysoidine which was begun 

 on May 21st [vide p. 158). 



Exp. 30. Monkey 7. — [Cercopithents mhaux). Jlay 15th. Inoculated subcutaneously 



K 



