TBYPANOSOMIASIS IN THE ANGLO-EGYPTIAN SUDAN 155 



Exp. 2. Dog 2. — Fefiriian/ 12t/i. Weight 9 kilos. About 80 trypanosomes per field, 

 1 c.c. sat. sol. Chrysoidine (•! gram in 10 c.e. aq. dest.) injected subcutaneously. 



February 13^/;. 2 c.c. 



February lAith. 2"5 c.c. Animal livelier. 



February l&h. 2-5 c.c. 



February liith. 2"5 c.c. Blood examined. Only two trypanosomes per field seen at 

 the most. Many fields ban*en. The parasites seemed to be rather sluggish, but no special 

 change was noted in them. 



February 11 th. 25 c.c. Animal ill and very weak. 



February Vith. 2'5 c.c. Blood examined, and found to be simply swarming with 

 trypanosomes which had greatly altered in appearance. Nearly all of them had become 

 more granular, and exhibited swollen posterior ends. In addition, curious involution forms 

 were present. One apparently unaltered form showed extreme activity, jerking and twisting 

 very violently, and having also a considerable movement of translation. Many forms were 

 sluggish, and a number became motionless forty-five minutes after the slide was prepared, 

 Agglutination on a small scale was also seen to take place about this time. A certain 

 number, all lung thin forms, remained active and lively. Further, dividing forms were 

 present, and many were seen united by their posterior ends. 



On staining, the above points were emphasized and vacuoles were found tu be present, 

 the vacuoloid area close to the centrosome being very well marked in the short forms. 



February 19t//. Dog distinctly better, though still very weak. 



Weight, 6-25 kilos. 



3'5 c.c. given. 



Blood much as above, but no agglutination forms seen. 



Long forms very active. 



The animal did not take food well in the afternoon, and appeared to be thirsty. 



February 20th. Found dead, stiS" and cold in the morning. 



The post-mortem has been mentioned (p. 126). Bacterial invasion had occurred, and no 

 trypanosomes were found in any of the smears. 



Exp. 6. Dog 3. February '11th. 5 c.c. sat. sol. given intravenously. 



February 28th. 1 c.c. sat. sol. given intravenously. 



March 1st. 2 c.c. sat. sol. given intravenously. 



March 2/1(1. 2'5 c.c. intravenously. 



No change in the dog's condition had been apparent. Owing to great press of work the 

 examination of the blood was put off from day to day, and the dog was found dying on 

 March 3rd. A post-mortem was performed immediately after death, and no trypanosomes 

 were found in anv of the smears made from heart's blood, liver, spleen and thymus gland, 

 which was enlarged. The urine was of a very deep yellow colour, and though I have no 

 proof of it, I am not at all certain but that the chrysoidine was the immediate cause of 

 death in this case. There was slight nephritis. I have, however, given a control dog 

 considerable doses, about 2 c.c. daily, intravenously, for a prolonged period without any ill 

 effects resulting. 



Exp. 8. Monkey 2. Weight, 2"5 kilos. February 21n^ Blood full of trypanosomes, 

 about 2U per field — -3 c.c. sat. sol. (1 gram in 10 c.c. aq. dest.) given subcutaneously. 



February 22nd. Animal seemed livelier. Only about six parasites per field. 

 3 c.c. given. 



Records of 

 cases 



