TRYPANOSOMIASIS IN THE ANGLO-EGYPTIAN SUDAN 159 



every day thereafter till June 19tli. About June 18th the animal began to show distinct 

 signs of weakness. Emaciation set in but there was very little somnolence. Towards the 

 end the trypanosomes increased in number in the peripheral blood. The monkey was found 

 dead on the morning of June 24th, and slight decomposition had occurred. The temperature 

 record during treatment is shown. Fig. 74. 



Por.t-mortem. The only marked change consisted in an ulcerated area at the lower end 

 of the ileum. It was not haemorrhagic in nature but there was a good deal of congestion 

 present. A smear was made but unfortunately was mislaid, and I have no notes of the 

 condition found. There was no gastric ulceration. The spleen was not enlarged nor was 

 the liver increased in size or congested. A smear was made of the semi-fluid contents of the 

 large gut but it shared the fate of the intestinal smear. 



As from results obtained with T. Gainhicuse by Dr. Neave it seemed possible that a 

 better result might be obtained by injecting the dye as a suspension ami in larger (juautities 

 than hitherto given, 



Exp. 46. Monkey 10 (Weight 3 kilos.) was conducted, beginning on the fifteenth day 

 after inoculation. 



October' nth. Blood full of trypanosomes. Both forms present. 



Octohi'r 19//i. 2 grains of Merck's chrysoidin given as a suspension by intramuscular 

 injection. 



Octohff 20th. Trine markedly yellow. Colour test with HCl. positive. Blood 

 swarming. Short forms showed vacuoles and many chromatin granules. 2 grains given. 



Ocfolicr 21st. Monkey very weak. Some rosettes of the short forms seen, and certain 

 of the long forms appeared to be degenerated. 2 grains (if. 13 gram) as above. 



October 22nd. Monkey very ill, the head held low and a general aspect of e.xtreme 

 depression presented. 2 grains given in the morning but the monkey died in the afternoon, 

 being the 18th day after inoculation. 



Pmt-mnrtem. — Spleen enlarged and very much congested. Smears made from this 

 organ showed a very remarkable state of affairs, in that many of the trypanosomes ha<l 

 almost completely broken down, nothing being left but the centrosomes, the free edges of the 

 undulating membranes and the flagella. Curious spherical and faintly stained "shadow" 

 forms were also present. So were vacuolated forms as well as apparently unaltered 

 trypanosomes. Sections were prepared for further study. 



The liver was enlarged and congested. There was no gastric or intestinal ulceration. 

 A smear from a congested area of gastric mucous membrane proved negative as regards the 

 presence of parasites. The brain and spinal-cord were slightly yellow. A smear of the 

 cerebro-spinal fluid exhibited the presence of unaltered trypanosomes in considerable 

 numbers. No streptococci were seen. 



In the bone-marrow some altered trypanosomes were present. Most of these were the 

 faintly staining and probably moribund " shadow" forms. They were not numerous. There 

 is little doubt but that the administration of chrysoidine hastened death in this case, though 

 whether the dye proved fatal by reason of its own toxic action on the tissues, or on account 

 of the great destruction of parasites, I cannot as yet say. No convulsions were seen, and it 

 will be noted that the trypanosomes found in the cerebro-spinal fluid were of normal aspect. 

 This case served to confirm mv view that the drug was of no value in infection with 

 T. dimorphiim, but a last trial was made in the following experiment. 



Exp. 49. ]\Ionkey 20 (weight 12 kilos) which had been inoculated on November 15th. 



