160 TRYPANOSOMIASIS IN THF. ANOLO-EOVrTI AN SITIAN 



Novfiiiliir 'l\Uh. IJotli furiiis |ins('iit an<l nimurous, some undergoing longitudinal 

 division. Monkey ill aiul eniaciuted. Given 2 grains Merck's ehrysoidin subcutaneously in 

 suspension. 



Sorniihei- W//i. Found in a curious, giddy, sttiporosc condition. Pupils slightly 

 contracted. The aiiiiiiul kept swaying to and fro and the head was held low. Though dull 

 and dazed it could be roused to attention. 



Blood examined. Some dead and " shadow " forms found. Dividing forms present. 

 No inarkcil disintegration. .'? grains given as above. Shortly after their ailiniiiistration the 

 animal <(dlapsed and lay upon its side in the cage. After tlie lapse of an hour as it was 

 apparently in extremis, chlorofonn was administered. 



Post-mortem . — Liver and spleen enlarged and congested. The latter organ had an old 

 puckered cicatrix running across its anterior surface. Some congestive patches were present 

 in the stomach, probably of a physiological nature. 



Intestines healthy. A small deep purple gliiml was fonml in the mesentery. Hrain, 

 spinal-cord and nerve trunks were all staineil an intense yellow colour. The cerebro- 

 spinal fluid was blood-stained but not yellow. 



Micrtiscopif e.iiuni nation. — Cerebro-spinal fluid. A fresh prcjiaration showed living 

 and dead forms of trypanosome. The former were sluggish, or speedily became so. 

 On staining, dead, altered, and degenerated forms were seen, as well as an unaltered 

 trypanosomcs. 



(ilanti .linear. — As above, and young forms as ilcscribed by Liiigard,* resembling 

 Leishman-Donovan bodies. 



/train .linear. — Some altii'ed anil nianv uualti reil f(inns. 



/.leer snwar. — Mostly unchanged forms. Some dead and altered forms. 



Sjileen smear. — Not so many trvpanosomes as in the liver smear, but a very large 

 proportion of the curious spherical and "shadow" forms showing segmented nuclei, 

 Centrosonies well marked. 



Jfear/s hloinl. — Most of the trvpanosomes staine<l well, and were unaltered. A few 



changed fnnns were present. Portions of the liver, spleen and brain were kept for .section 



work. 



Chnsoidinca It will be Seen that in the case of what is probably T. lUmorjihum, a trypanosome 



aiureint e i„lii|jttedlv verv resistant to anv of the known modes of treatment, chrvsoidine has proved a 



somiasisof failure. In no instance has it prolonged life, while on several occasions, when given in doses 



large enough to j)rofoundly aft'ect the parasites, death of the host has resulted. Two forms 



have been tested, nine cases in all have been treated, the dye has been given in varying 



doses, both in solution and in suspension, and though interesting effects have been observed, it 



cannot be said that these have proved of a beneficial nature. Dr. Chauvin of Mauritius 



writes me to say that he has tried chrysoidine there in horses suffering from Surra, but with 



no success whatever. Whether a eiind)ination of the dye with an arsenical preparation, such 



as has been emidoved along with trvpan re(I, would \ield better results, I cannot say. 



Time has not admitted of a trial of this nature. 



As already mentioned. Dr. NeavefJ had an opportunity of testing chrysoidine on a case of 

 human trypanosomiasis from Uganda, in the case of the boy Wariga mentioned in his report. 



• Iiidinn Med. Oazette, Calcutta, \^;>, Sept. Vol. il., pp. .3.33 et $tq. and 381. 

 t LniH-et, Londiin, June 13th, 190,1, p, l.f>4.'l. 

 X For Dr. Nciive's accouut of the case, sec p. 18."). 



mules 



