TRYPANOSOMIASIS IN THE ANGLO-EGYPTIAN SUDAN 167 



April Sth. A lessening in tlie number of trypanosomes noted. Mostly short forms 

 present, which were sluggish. On staining, these were found to show many granules, and 

 to possess vacuoles. 2'5 c.c. given. 



April 2th. Not a single trypanosome found, either in fresh or stained preparations. 

 2'5 c.c. given. 



April lOt/i. Monkey well. Shows no sign of the disease. A few trypanosomes found. 



April llf.h. Condition much the same. Three distinct forms observed, (a) Typical 

 long forms. {l>) Typical short forms. (o) Intermediate forms with rather short flagella. 

 A few involution forms, some with swollen posterior ends. 3 c.c. given. The long forms, 

 some of which were in process of division, all appeared to be of the same kind as regards 

 general shape, length of flagella and size of eentrosomes and nuclei. There was nothing to 

 lead one to suppose that male sexual forms were present in the blood. The intermediate 

 forms (c) were probably merely some of the usual short forms in which the flagella were more 

 developed than is customary. None of the short forms were dividing, and in no case was the 

 flagellum of any great length. 



Ajiril 12th. No change. 4 c.c. given. 2 c.c. in morning, 2 c.c. in afternoon. 



April ISl/i. No change. 4 c.c. given. 



April lifh. Only 3 c.c. given in one dose to-day. 



Apiril 15th. 5 or 6 lively trypanosomes per field. Appearance as of " tadpole " forms. 

 4 c.c. given in two doses. 



April li\tlt. No change. Mostly long forms in blood. 6 c.c. given in two doses. 



April nth. In fresh film one sluggish long form found after a long search. In stained 

 preparation some short forms evident. Most seen three per field. G c.c. given. 



April 18th. Much as above but more numerous, there being as many as 12 in one field. 

 (Leitz, Oc. 4, oil imm., iVth.) 



April 12ih. No change. No increase. Vacuoles very apparent. 8 c.c. given 

 in two doses. 



April 20fh. In fresh film no parasites found after a long search. In stained 

 preparation a few unaltered forms found after a considerable hunt for them. Animal well. 

 Weight 2 "6 kilos, being a gain of '2 of a kilo from commencement of experiment. 

 8 c.c. given. 



April 2lst. A single morning dose of 6 c.c. given. 



April '22ml. No change. 10 c.c. given, in two doses. 



Ap>ril 2?>ril. Trypanosomes as numerous as ever. 10 c.c. given. 



April 21th. Trypanosomes apparently ■ increasing in number. 12 c.c. given. The 



serum, which had acquired a peculiar odour, had probably undergone some change, and 



-» become inert. Treatment was accordingly discontinued. Thereafter a steady increase took 



place in the number of parasites, and the monkey began to lose weight (2 kilos on 29th) and 



go down-hill. 



Mill/ 1st. Found dead. Bacterial invasion. An ulcer was found in the cfficum. No 

 erosion of stomach. Liver and spleen not markedly congested. Mesenteric glands 

 enlarged. 



Smears from spleen, liver, lung, bone-marrow, and glands negative, owing to bacterial 

 invasion. 



Exp. 18. Gerbil 11. March 29th. Both forms present and numerous. 10 m. given 

 subcutaneously. 



