168 TUYl-ANOSOMlAslS IN THK ANOLO-KGYPTIAN HIDAN 



Miirr/i .'Ul/Zi. Trypiiiiosomcs more numerous. Long typical forms, tliiek intermediate 

 forms, without flugellii ami blunt, or somewhat pointed at posterior ends, and tiny short forms 

 seen. 'I'lic distinctions were well marked. 15 m. given. 



Mtirc/i 3lsr. l.*) m. given. No change. 



April l.it. 20 m. given. No change. 



Ajiril 'Inil. "iU m. given. Animal very ill. 



A/trll SnI. Fouiiij ilciiil. Altliipiij^li 11(1 iibscess formiitiiin IlhI (niiuiiil. fiDin tlic 

 state of the tissues round tlic site of inoeuhition I am inclined to think this animal may 

 liave died of sepsis. The post-mortem results have been reporti'd. Nothing was found save 

 a bacterial invasion of the tissues. 



Exp. 25. Monkey 6. Weight 13 kilos. 



.Vai/ 5tli. Trypanosomes numerous, 2U or 'W per field. Both forms present. Long 

 forms in process of division. 



.)/(((/ (U/i. On this date, being the thirtccntli diiv after inoculation, 7 c.c. of blood 

 serum nf water-buck of date January 27th, 1905, wen- injected subciitaneously. lilooil taken 

 5 hours after inoculation. Fresh blood swarming with very active parasites. Animal's 

 condition unchanged. On staining, it was found that, wliile the majority of the trypanosomes 

 Were unclianged, some hail completely broken down, their centrosomes, free edges of tlie 

 undulating membranes and llagella alone persisting. .Many curious forms, some with huge 

 undulating membranes were seen. Vacuolated and ■ sliadow " forms present. Short forms 

 chiefly art'eeted. 



Mill/ "till. JMood exaniiiU'(l <luriiig the afternoon. Nearly every trypaiiosome much 

 vacuolated. Not so many curious degenerated (?) or involution fnrnis present but a 

 greater number of trypanosomes affected to some extent. No change in general condition of 

 monkey. Given 10 c.c. blood serum at 4.15. Blood taken at 5.5 p.m. No change. 



AjirilBf/i. Fresh lilm showed living and active tryj)auosomes of both forms. Long 

 forms do not now appear to be affected. r2'5 c.c. given. Blood examined 4i hours after 

 inoculation. Extreme vacuolation of short forms noticed. Some of the [parasites seemed 

 almost to consist of vacuoles united by thin strands of cytoplasm. Many greatly 

 degenerated forms (Plate XIV., Fig. '/). 



Afdif 9th. Blood in much the same condition. Some lo.ss in differential staining as 

 regards the parasites. About 19 c.c. given in four separate doses. After half an hour the 

 monkey was evidently suffering from giddiness. It ap))eared to be ill. and made strange, 

 aindess efforts to climb up the side of its cage. Some tremors were ob-served, together 

 with a transient rigidity of the hind legs. No twitching of face or fixity of gaze. The 

 unimul several times fell over on its si<Ie but (juickly recovered itscdf. This condition of 

 affairs lasteil for 1^ hours. Thereafter recovery took place and the monkey took food 

 freely. The blood examined during the attack and also 4i hours after the inoculation 

 showed no change in the state of the trypanosomes. The attack was probably due 

 to the large amount of fluid which was given causing a disturbance in the semi-circular 

 canals. 



Mii;i [(Uh. Small rosettes of trypanosomes found, many resembling the I^Ianx Coat-of- 

 Arms. Dividing forms present. On staining, degenerate or dead forms were seen. The 

 monkey was better and took its food well, but was somewhat somnolent, ami kept its head 

 down as if depressed. 



Mat/ lllli. Serum treatment stopped. From this time the monkey rapidly went down- 



